To better comprehend the relationship between left/right (L/R) differences and hippocampus functions is necessary knowledge of lateral asymmetry and regional distribution. This research was design to examine hippocampal L/R asymmetry and regional distribution profile of the alpha7 and alpha4 subtypes of nicotinic acetylcholine receptors (nAChRs) in the adult rat.

10–12-week-old twenty-four male wistar rats were randomly selected. After removing the brains, immunohistochemistry, real-time PCR, and western blot methods were applied to distinguish the presence of the receptors in the hippocampus. Outcomes stated that the mentioned receptors expression profile was spatial-dependent. As, the hippocampal dispersal of alpha7 and alpha4 subtypes in the left hippocampus (LH) was remarkably maximum compare with the right hippocampus (RH) (p = 0.001, p = 0.005 respectively). Furthermore, the alpha7 optical density (OD) was not significantly different in the diverse regions in hippocampus of adult rat (p = 0.057), while the maximum OD of the alpha4 was detected in the hippocampal dentate gyrus and CA3 regions of LH (p = 0.007, p = 0.009 respectively) and the minimum OD was in the CA1 of the RH (p = 0.019). In real time PCR evaluation, there is a significantly higher expression of alpha7 and alpha4 in LH compared to RH (p = 0.043, p = 0.049 respectively), also, for western blot (p = 0.042, p = 0.030 respectively). According to present data, the alpha7 and alpha4 nAChR subtypes expression profile demonstrated lateral asymmetry, the uniform regional dispersal for alpha7 and different regional dispersal for alpha4 in the adult rat hippocampus.

为了更好地理解左右 (L/R) 差异与海马功能之间的关系，需要了解横向不对称和区域分布。本研究旨在检查成年大鼠海马 L/R 不对称性和 alpha7 和 alpha4 亚型烟碱乙酰胆碱受体 (nAChRs) 的区域分布概况。

随机选择 10-12 周大的 24 只雄性 wistar 大鼠。取出大脑后，应用免疫组织化学、实时 PCR 和蛋白质印迹方法来区分海马中受体的存在。结果表明，上述受体表达谱是空间依赖性的。因为，与右侧海马（RH）相比，左侧海马（LH）中α7和α4亚型的海马扩散显着最大（分别为p = 0.001，p = 0.005）。此外，成年大鼠海马不同区域的α7光密度（OD）没有显着差异（p = 0.057），而在LH的海马齿状回和CA3区域检测到α4的最大OD（p = 0.007，p = 0.009），最小 OD 位于 RH 的 CA1 中（p = 0.019）。在实时 PCR 评估中，与 RH 相比，LH 中 alpha7 和 alpha4 的表达显着更高（分别为 p = 0.043，p = 0.049），对于蛋白质印迹（分别为 p = 0.042，p = 0.030）。根据目前的数据，成年大鼠海马中 alpha7 和 alpha4 nAChR 亚型表达谱表现出横向不对称、alpha7 的均匀区域分散和 alpha4 的不同区域分散。