Collagen as a biomaterial is commonly obtained from terrestrial animals. However, nowadays, the use of collagen with terrestrial animals' sources due to possible transmission of infectious diseases and religious beliefs is restricted. This study was conducted to evaluate the physicochemical characterization, morphology, and biocompatibility of extracted collagen from silver carp fish skin by-product. Type I collagen was extracted from the silver carp fish skin by-product using acetic acid and pepsin enzyme. The results showed that the extraction yield of ASC and PSC was 42.85% and 58.75% (on a dry weight basis), respectively. The presence of two different α-chains confirmed the type I collagen structure for both collagens. Moreover, FTIR spectra investigation demonstrated the triple helical in ASC and PSC collagens. The PSC showed higher imino acids content than ASC. Hence, the fractional viscosity and DSC curves revealed higher T_d (30 °C), T_s (81 °C), and T_m (209 °C) for PSC than ASC (28.73 °C, 77 °C, and 187 °C, respectively). Finally, observations of the microscopic and the cell viability evaluation confirmed biocompatibility and suitable structure to cell growth. Accordingly, the obtained collagen from silver carp fish skin can be a proper alternative for terrestrial animals’ collagen and a safe biomaterial for biomedical use.

胶原蛋白作为生物材料通常从陆生动物获得。然而，如今，由于传染病和宗教信仰的可能传播，限制了在陆生动物来源中使用胶原蛋白。进行这项研究以评估从silver鱼鱼皮副产物中提取的胶原蛋白的理化特性，形态和生物相容性。使用乙酸和胃蛋白酶从from鱼鱼皮副产物中提取I型胶原。结果表明，ASC和PSC的提取率分别为42.85％和58.75％（以干重计）。两条不同的α链的存在证实了两种胶原蛋白的I型胶原蛋白结构。此外，FTIR光谱研究表明ASC和PSC胶原中的三螺旋。PSC显示出比ASC更高的亚氨基酸含量。因此，分数粘度和DSC曲线显示较高PSC的T _d（30°C），T _s（81°C）和T _m（209°C）比ASC（分别为28.73°C，77°C和187°C）。最后，显微镜观察和细胞活力评估证实了生物相容性和适合细胞生长的结构。因此，从silver鱼鱼皮获得的胶原蛋白可以作为陆生动物胶原蛋白的合适替代物，并且是用于生物医学的安全生物材料。