Coherent anti-Stokes Raman scattering (CARS) spectroscopy is a powerful tool for Raman imaging technology. In contrast, conventional spontaneous Raman spectroscopy is often used for biological analysis with multivariate analysis. This study develops a new type of CARS instrument with a double-wavelength-emission background-free electronically tuned Ti:sapphire laser (DW-ETL). DW-ETL generates two laser pulses with different wavelengths simultaneously within its single resonator. The pulse wavelength and buildup time are regulated by acousto-optical tunable filter (AOTF) in the resonator. The present DW-ETL CARS system is free from any mechanical movement to measure a CARS spectrum by controlling each laser pulse of the emission throughout the fingerprint region. Consequently, it is theoretically able to provide stable CARS spectra to apply multivariate analysis in biological applications. The present study demonstrates that the DW-ETL CARS system provides spectra of biomedical samples in the full finger-print region, and the stability and controllability of the system are evaluated.

相干反斯托克斯拉曼散射 (CARS) 光谱是拉曼成像技术的强大工具。相比之下，传统的自发拉曼光谱通常用于多变量分析的生物分析。本研究开发了一种具有双波长发射无背景电子调谐钛蓝宝石激光器 (DW-ETL) 的新型 CARS 仪器。DW-ETL 在其单个谐振腔内同时产生两个不同波长的激光脉冲。脉冲波长和建立时间由谐振器中的声光可调滤波器 (AOTF) 调节。本 DW-ETL CARS 系统通过控制整个指纹区域发射的每个激光脉冲，无需任何机械运动来测量 CARS 光谱。最后，理论上能够提供稳定的CARS光谱，以在生物应用中应用多元分析。本研究表明，DW-ETL CARS 系统提供了全指纹区域的生物医学样本光谱，并对系统的稳定性和可控性进行了评估。