Abstract

Pseudopyrenochaeta lycopersici is a soil-borne fungus causing corky root of tomato. The slow growth and poor sporulation of the pathogen make its isolation difficult. Isolates of the fungus are classified into types 1 and 2, with many different physiological and molecular characteristics between both types. In general, mating type genes enable mating ability and sexual development. The type 1 strains CBS267.59 and Oha3-6 of P. lycopersici were utilized to amplify DNA binding domains (HMG box). The MAT1-1-1 gene of CBS267.59 encodes a predicted protein including an alpha box DNA binding domain, while MAT1-2-1 of Oha3-6 encodes a predicted protein including an HMG DNA binding domain. The sequences of the putative P. lycopersici α and HMG boxes were very similar to established α and HMG boxes. ORF1 and the DNA lyase gene were found in the 5ʹ and 3ʹ regions, respectively, of the MAT1-1 locus. Comparison of the mating type genes and flanking regions was carried out between the strains CBS267.59 and Oha3-6 and other Loculoascomycetes, Pyrenomycetes and Discomycetes. The assembled sequences were 2932 bp (nucleotide positions 426–3358) for the MAT1-1 idiomorph and 3410 bp (nucleotide positions 438–3848) for the MAT1-2 idiomorph. Multiplex polymerase chain reaction amplified 432 bp and 786 bp bands in the MAT1-1 and MAT1-2 strains, respectively. The isolation and characterization of the MAT genes of P. lycopersici improve our knowledge and may help to explain the taxonomic differences in types 1 and 2 of this fungus.

摘要

Pseudopyrenochaeta lycopersici是一种土传真菌，导致番茄软木根。病原体的生长缓慢和孢子形成不良使其分离困难。真菌的分离物分为 1 型和 2 型，两种类型之间具有许多不同的生理和分子特征。一般来说，交配型基因使交配能力和性发育成为可能。P. lycopersici 的1 型菌株 CBS267.59 和 Oha3-6用于扩增 DNA 结合域（HMG 盒）。所述MAT1-1-1 CBS267.59的基因编码的预测的蛋白包含α-盒DNA结合结构域，而MAT1-2-1 Oha3-6的编码的预测蛋白质包含HMG DNA结合结构域。假定的P. lycopersici的序列α 和 HMG 盒与已建立的 α 和 HMG 盒非常相似。ORF1和DNA 裂解酶基因分别位于MAT1-1基因座的 5ʹ 和 3ʹ 区域。在菌株CBS267.59和Oha3-6与其他腔囊菌纲、核菌纲和盘状菌纲之间进行交配型基因和侧翼区域的比较。组装的序列是用于2932 bp的（核苷酸位置426-3358）MAT1-1用于idiomorph和3410碱基对（核苷酸位置438-3848）MAT1-2 idiomorph。多重聚合酶链反应在MAT1-1和MAT1-2 中扩增了 432 bp 和 786 bp 条带株，分别。P. lycopersici MAT基因的分离和表征提高了我们的知识，并可能有助于解释这种真菌 1 型和 2 型的分类差异。