ABSTRACT

The determination of ovarian stages is essential for an understanding of reproductive biology and informing sustainable management of commercially caught marine organisms. This research developed improved microscopic assessment criteria for the prawn, Penaeus (Melicertus) latisulcatus, by reviewing microscopic criteria used in the literature and assessing cell composition of ovary tissue from 97 females. Whilst histological staging is not a new technique, this research outlines new development criteria for assessing ovary development stages in P. latisulcatus. A principal component analysis (PCA) was used with the microscopic classification system and showed distinct groups for each stage. Based on the proportion of cell types, distinct groupings were apparent, which corresponded to the main microscopic stages, in particular for stage 3 ovaries. This revised classification will improve the accuracy of ovarian staging techniques for P. latisulcatus and can be used as a guide for the interpretation and analysis of macroscopic stages during seasonal spawning events for the purpose of better informing fisheries management.

摘要

确定卵巢阶段对于了解生殖生物学和为商业捕获的海洋生物的可持续管理提供信息至关重要。本研究通过回顾文献中使用的微观标准并评估来自 97 只雌性的卵巢组织的细胞组成，制定了改进的对虾的微观评估标准，Penaeus (Melicertus) latisulcatus。虽然组织学分期不是一项新技术，但本研究概述了评估P. latisulcatus卵巢发育阶段的新发展标准. 主成分分析 (PCA) 与微观分类系统一起使用，并显示每个阶段的不同组。根据细胞类型的比例，不同的分组是明显的，这对应于主要的微观阶段，尤其是第 3 阶段的卵巢。这一修订后的分类将提高P. latisulcatus卵巢分期技术的准确性，并可用作解释和分析季节性产卵事件期间宏观阶段的指南，以便更好地为渔业管理提供信息。