Plasma biochemical analysis remains one of the established ways of monitoring captive marine mammal health. More recently, complementary plasma lipidomic analysis has proven to be a valid tool in disease diagnosis and prevention, with the potential to validate and complement common biochemical analysis, providing a more integrative approach. In this study, we thoroughly characterized the plasma polar lipid content of Tursiops truncatus, the most common cetacean species held under human care. Our results showed that phosphatidylcholine, lysophosphatidylcholine, and sphingomyelins (CerPCho) are the most represented phospholipid classes in T. truncatus plasma. Palmitic, oleic, and stearic acids are the major fatty acid (FA) present esterified to the plasma polar lipids of this species, although some n-3 species are also remarkably present, namely eicosapentaenoic and docosahexaenoic acids. The polar lipidome identified by HILIC LC–MS allowed identifying 304 different lipid species. These species belong to the phosphatidylcholine (103 lipid species), lysophosphatidylcholine (35), phosphatidylethanolamine (71), lysophosphatidylethanolamine (20), phosphatidylglycerol (13), lysophosphatidylglycerol (5), phosphatidylinositol (15), lysophosphatidylinositol (3), phosphatidylserine (6) lysophosphatidylserine (1), and sphimgomyelin (32) classes. This was the first time that the dolphin plasma phospholipid profile was characterized, providing a knowledge that will be important to further understand lipid metabolism and physiological regulation in small cetaceans. Furthermore, this study proved the practicability of the use of plasma lipid profiling for health assessment in marine mammals under human care.

中文翻译：

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Tursiops truncatus 的血浆磷脂组：从生理洞察到设计用于管理鲸目动物监测的前瞻性工具

血浆生化分析仍然是监测圈养海洋哺乳动物健康的既定方法之一。最近，补充血浆脂质组学分析已被证明是疾病诊断和预防的有效工具，有可能验证和补充常见的生化分析，提供更综合的方法。在这项研究中，我们彻底描述了Tursiops truncatus的血浆极性脂质含量，这是人类照料下最常见的鲸类物种。我们的研究结果表明，磷脂酰胆碱、溶血磷脂酰胆碱和鞘磷脂 (CerPCho) 是T. truncatus中最具代表性的磷脂类别等离子体。棕榈酸、油酸和硬脂酸是与该物种的血浆极性脂质酯化的主要脂肪酸 (FA)，尽管一些n-3 种也显着存在，即二十碳五烯酸和二十二碳六烯酸。HILIC LC-MS 鉴定的极性脂质组允许鉴定 304 种不同的脂质种类。这些种类属于磷脂酰胆碱（103 种脂质种类）、溶血磷脂酰胆碱（35）、磷脂酰乙醇胺（71）、溶血磷脂酰乙醇胺（20）、磷脂酰甘油（13）、溶血磷脂酰甘油（5）、磷脂酰肌醇（15）、溶血磷脂酰肌醇（3）、磷脂酰) 溶血磷脂酰丝氨酸 (1) 和鞘磷脂 (32) 类。这是首次对海豚血浆磷脂谱进行表征，为进一步了解小型鲸类的脂质代谢和生理调节提供了重要的知识。此外，