Ferrer-Gago & Koh report a method for the synthesis of complex proteins by native chemical ligation (NCL). The attachment of o-phenylenediamine (PheDA), with and without an electron withdrawing group, to a chlorotrityl chloride resin converts one amino group of PheDA to a secondary amine. This changes the reactivity of the two amino groups, enabling synthesis of o-aminoanilide peptides without overacylation of the additional unprotected amino group. PheDA attached to the trityl resin allows the incorporation of glycine in only one amino group, facilitating the synthesis of C-terminal glycine-rich o-aminoanilide peptides. After treatment with sodium nitrite, these form acyl-benzotriazole active esters. The esters undergo a thiol exchange yielding thioester peptides used in NCL reactions. (doi: 10.1002/pep2.24194)

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Ferrer-Gago＆Koh报告了一种通过天然化学连接（NCL）合成复杂蛋白质的方法。具有和不具有吸电子基团的邻苯二胺（PheDA）与氯三苯甲基氯树脂的连接将PheDA的一个氨基转化为仲胺。这改变了两个氨基的反应性，使得能够合成邻氨基苯胺肽而不会另外的未保护的氨基过度酰化。PheDA附着于三苯甲基树脂允许甘氨酸掺入仅一个氨基中，促进C末端的富含甘氨酸的合成Ò-氨基苯胺肽。用亚硝酸钠处理后，它们形成酰基-苯并三唑活性酯。酯进行硫醇交换，产生用于NCL反应的硫酯肽。（doi：10.1002 / pep2.24194）