Gram-negative binding proteins (GNBPs) are important in the innate immune system of insects in recognition of fungi pathogen, such as Beauveria bassiana. However, this information in aphids is not clear, which might be exploited to develop a novel aphid control strategy based on integrating RNAi and B. bassiana. Here, we firstly identified two GNBPs, ApGNBP1 and ApGNBP2, using the model aphid Acyrthosiphon pisum, and observed that two ApGNBPs were highly expressed in hemolymph and fat body as well as upon the injection of β-1,3-glucan and heat-killed B. bassiana. Intriguingly, RNAi-based silencing of ApGNBP1 but not ApGNBP2 decreased the activity of immune-related phenoloxidase. This led to the increased virulence of B. bassiana in A. pisum upon silencing of ApGNBP1, and the synergetic effects were also observed in other two aphids: Myzus persicae and Aphis citricidus. Importantly, no negative effects were detected in aphid predator Propylaea japonica under the co-application of the RNAi in targeting ApGNBP1 and B. bassiana. Taking together, we conclude that GNBP1 could be an optimal target in aphid control by combining RNAi, entomopathogenic fungi and ladybeetle predator.

革兰氏阴性结合蛋白（GNBP）在昆虫的先天免疫系统中对于识别真菌病原体（如球孢白僵菌）很重要。但是，关于蚜虫的信息尚不清楚，可以利用该信息来开发基于RNAi和B整合的新型蚜虫控制策略。鲈鱼。在这里，我们首先使用蚜虫Acyrthosiphon pisum模型鉴定了两个GNBP，分别为ApGNBP1和ApGNBP2，并观察到两个ApGNBP在血淋巴和脂肪体内以及注射β-1,3-葡聚糖和加热后高表达-killed乙。鲈鱼。有趣的是，基于RNAi的ApGNBP1沉默而不是ApGNBP2沉默降低了免疫相关酚氧化酶的活性。这导致B. bassiana在A中的毒力增加。豌豆时的沉默ApGNBP1，并且协同作用在其它两种蚜虫中也观察到：桃蚜和棉蚜蚜。重要的是，在RNAi共同靶向ApGNBP1和B的条件下，蚜虫天敌Propylaea japonica没有发现负面影响。鲈鱼。综上所述，我们得出结论，GNBP1 通过组合RNAi，昆虫病原真菌和瓢虫捕食者，可能成为蚜虫控制的最佳靶标。