ABSTRACT

Environmental risk factors are recognized as threats to public health. Stress granules (SGs) are non-membranous assemblies of mRNAs and proteins expressed in response to various stressors to promote cell survival. In this study, SG formation was examined to confirm the effects of polyhexamethylene guanidine phosphate (PHMG), chloromethylisothiazolinone (CMIT), and particulate matter (PM10) in airway epithelial cells, A549, HPAEpiC, and BEAS-2B cells. SGs were not observed after CMIT, PHMG, and PM10 treatments, as determined by immunofluorescence microscopy. Moreover, there was no change in the phosphorylation of the translation initiation factor eIF2αfollowing treatment with PHMG, CMIT, and PM10. Taken together, our findings might help determine the biological hazards of these materials.

摘要

环境风险因素被认为是对公众健康的威胁。压力颗粒 (SG) 是 mRNA 和蛋白质的非膜组装体，它们响应各种压力源而表达，以促进细胞存活。在这项研究中，研究了 SG 形成以确认聚六亚甲基胍磷酸盐 (PHMG)、氯甲基异噻唑啉酮 (CMIT) 和颗粒物 (PM10) 在气道上皮细胞、A549、HPAEpiC 和 BEAS-2B 细胞中的影响。通过免疫荧光显微镜确定，在 CMIT、PHMG 和 PM10 处理后未观察到 SG。此外，在用 PHMG、CMIT 和 PM10 处理后，翻译起始因子 eIF2α 的磷酸化没有变化。总之，我们的发现可能有助于确定这些材料的生物危害。