SummaryTo determine the effects of sperm DNA fragmentation (SDF) on embryo morphokinetic parameters, cleavage patterns and embryo quality, this retrospective study analyzed 151 intracytoplasmic sperm injection (ICSI) cycles (1152 embryos collected) between November 2016 and June 2019. SDF was assessed using sperm chromatin dispersion. The cycles were divided into two groups based on the SDF rate: SDF < 15% (n = 114) and SDF ≥ 15% (n = 37). The embryo morphokinetic parameters, cleavage patterns, and embryo quality were compared between the two groups. The morphokinetic parameters tPNf, t2, t3, t4, t5, t6, and t8 were achieved significantly earlier in the SDF < 15% group compared with in the SDF ≥ 15% group. The fertilization and 2PN rates seemed to be significantly higher in the SDF < 15% group compared with in the SDF ≥ 15% group, while the abnormal cleavage rates were similar. However, a significantly higher rate of chaotic cleavage (CC) was observed in the SDF ≥ 15% group. The D3 high-quality embryo and available embryo rates were similar between the two groups. The blastocyst formation, high-quality blastocyst, and available blastocyst rates in the SDF < 15% group were significantly higher than those in the SDF ≥ 15% group. With an increase in SDF level, the chemical pregnancy, clinical pregnancy and implantation rates tended to decrease, while the miscarriage rate increased. This study demonstrated that SDF ≥ 15% reduces the fertilization rate of ICSI cycles and affects certain morphokinetic parameters. A higher SDF level can also induce a higher rate of CC, with subsequent decreases in the blastocyst formation rate and blastocyst quality.

中文翻译：

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通过精子染色质分散测量的精子 DNA 片段化影响 ICSI 治疗中的形态动力学参数、受精率和囊胚质量

摘要为了确定精子 DNA 片段化 (SDF) 对胚胎形态动力学参数、卵裂模式和胚胎质量的影响，这项回顾性研究分析了 2016 年 11 月至 2019 年 6 月期间的 151 个胞浆内精子注射 (ICSI) 周期（收集了 1152 个胚胎）。精子染色质分散。根据 SDF 率将周期分为两组：SDF < 15% (n= 114) 和 SDF ≥ 15% (n= 37)。比较两组的胚胎形态动力学参数、卵裂模式和胚胎质量。与 SDF ≥ 15% 组相比，SDF < 15% 组的形态动力学参数 tPNf、t2、t3、t4、t5、t6 和 t8 明显更早实现。与 SDF ≥ 15% 组相比，SDF < 15% 组的受精率和 2PN 率似乎显着更高，而异常卵裂率相似。然而，在 SDF ≥ 15% 组中观察到显着更高的混沌分裂 (CC) 率。两组的D3优质胚胎率和可用胚胎率相似。SDF < 15% 组的囊胚形成率、优质囊胚率和可用囊胚率明显高于SDF ≥ 15% 组。随着 SDF 水平的增加，化学妊娠率、临床妊娠率和着床率呈下降趋势，流产率呈上升趋势。该研究表明，SDF ≥ 15% 会降低 ICSI 周期的受精率并影响某些形态动力学参数。更高的 SDF 水平也可以诱导更高的 CC 发生率，随后胚泡形成率和胚泡质量下降。