The bean bug (Riptortus pedestris) causes great economic losses of soybeans by piercing and sucking pods and seeds. Although R. pedestris has become the focus of numerous studies associated with insect–microbe interactions, plant–insect interactions, and pesticide resistance, a lack of genomic resources has limited deeper insights. Here, we report the first R. pedestris genome at the chromosomal level using PacBio, Illumina, and Hi-C technologies. The assembled genome was 1.080 Gb in size with a contig N50 of 2.882 Mb. More than 96.3% of the total genome bases were successfully anchored to six unique chromosomes. Genome resequencing of male and female individuals and chromosomic staining demonstrated that the sex chromosome system of R. pedestris is XO, and the shortest chromosome is the X chromosome. In total, 19,026 protein-coding genes were predicted, 18,745 of which were validated as being expressed. Temporospatial expression of R. pedestris genes in six tissues and 37 development stages revealed 4,657 and 7,793 genes mainly expressed in gonads and egg periods, respectively. Evolutionary analysis demonstrated that R. pedestris and Oncopeltus fasciatus formed a sister group and split ∼80 million years ago (Mya). Additionally, a 5.04 Mb complete genome of symbiotic Serratia marcescens Rip1 was assembled, and the virulence factors that account for successful colonization in the host midgut were identified. The high-quality R. pedestris genome provides a valuable resource for further research, as well as for the pest management of bug pests.

中文翻译：

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豆虫 Riptortus pedestris 的染色体水平基因组组装

豆虫（Riptortus pedestris）通过刺吸豆荚和种子，给大豆造成巨大经济损失。虽然R . pedestris已成为与昆虫-微生物相互作用、植物-昆虫相互作用和农药抗性相关的众多研究的焦点，但缺乏基因组资源限制了更深入的了解。在这里，我们报告第一个R。行人使用 PacBio、Illumina 和 Hi-C 技术在染色体水平上分析基因组。组装的基因组大小为 1.080 Gb，重叠群 N50 为 2.882 Mb。超过 96.3% 的总基因组碱基被成功锚定在六个独特的染色体上。男性和女性个体的基因组重测序和染色体染色表明R的性染色体系统。pedestris是XO，最短的染色体是X染色体。总共预测了 19,026 个蛋白质编码基因，其中 18,745 个被验证为表达。R 的时空表达。行人6 个组织和 37 个发育阶段的基因分别揭示了 4,657 和 7,793 个主要在性腺和卵期表达的基因。进化分析表明，R . pedestris和Oncopeltus fasciatus形成了一个姐妹群，并在大约 8000 万年前分裂（Mya）。此外，还组装了共生粘质沙雷氏菌Rip1的 5.04 Mb 完整基因组，并确定了在宿主中肠中成功定植的毒力因子。高质量的R . pedestris基因组为进一步研究以及昆虫害虫的害虫管理提供了宝贵的资源。