Intermittent hypoxia (IH) is associated with the pathogenesis of necrotizing enterocolitis (NEC). We tested the hypothesis that early supplementation with antioxidants and/or fish oil protects the terminal ileum from oxidative injury induced by neonatal IH. Newborn rats were exposed to neonatal IH from birth (P0) until P14 during which they received daily fish oil, coenzyme Q10 (CoQ10), glutathione nanoparticles (nGSH), fish oil + CoQ10, or olive oil. Pups were then placed in room air from P14 to P21 with no further supplementation. Terminal ileum was assessed for IH-induced injury and inflammatory biomarkers. Neonatal IH induced severe damage consistent with NEC, and was associated with oxidative stress and elevations in PGE₂, PGF_2α, TxB₂, NOS-2 and TLR-4, effects that were ameliorated with nGSH and combination CoQ10+fish oil. Early postnatal supplementation with antioxidants and/or fish oil during neonatal IH may be favorable for preserving gut integrity and reducing oxidative injury.

间歇性缺氧（IH）与坏死性小肠结肠炎（NEC）的发病机制有关。我们测试了这样的假设：早期补充抗氧化剂和/或鱼油可以保护回肠末端免受新生儿 IH 引起的氧化损伤。新生大鼠从出生 (P0) 到 P14 均暴露于新生 IH，在此期间每天接受鱼油、辅酶 Q10 (CoQ10)、谷胱甘肽纳米颗粒 (nGSH)、鱼油 + CoQ10 或橄榄油。然后将幼崽从 P14 到 P21 放置在室内空气中，没有进一步补充。评估回肠末端 IH 诱导的损伤和炎症生物标志物。新生儿 IH 引起与 NEC 一致的严重损伤，并与氧化应激和 PGE ₂ 、PGF _2α 、TxB ₂ 、NOS-2 和 TLR-4 升高相关，nGSH 和 CoQ10+鱼油组合可改善这些影响。新生儿 IH 期间产后早期补充抗氧化剂和/或鱼油可能有利于保持肠道完整性和减少氧化损伤。