The bioactive constituents of sea cucumber Holothuria atra were investigated, and four sulfated holostan-type triterpene glycosides (1–4) along with other seven know metabolites were obtained. On the basis of the extensive NMR and ESI-MS data, the structures of triterpenoid saponins were assigned as a new compound echinoside B 12-O-methyl ether (1), and known echinoside B (2), 24-dehydroechinoside B (3), and holothurin B (4). The extract and isolated saponins displayed weak antioxidant DPPH radical scavenging activities, where echinoside B (2) showed the highest value at 26.41%. However, 24-dehydroechinoside B (3) showed mild antioxidant-reducing power activity (0.19 nm), compared to the synthetic antioxidant (BHT). Both compounds 2 and 3 exhibited moderate cytotoxic activity by reducing the viability of Ehrlich ascites carcinoma cells to 41.0% and 42.2%, respectively, compared to the drug control vincristine (95.2%).

海参的生物活性成分黑海参进行了调查，和四个硫酸holostan型三萜糖苷（1 - 4）获得与七种知道的代谢物一起。根据广泛的NMR和ESI-MS数据，将三萜皂苷的结构指定为一种新的化合物蛇毒苷B 12- O-甲基醚（1），以及已知的蛇毒苷B（2），24-脱氢蛇毒苷B（3）和全脂蛋白B（4）。提取物和分离出的皂苷显示出弱的抗氧化剂DPPH自由基清除活性，其中ech子苷B（2）的最高值为26.41％。然而，与合成抗氧化剂（BHT）相比，24-dehydroechinoside B（3）表现出轻度的抗氧化剂降低能力（0.19 nm）。与药物对照长春新碱（95.2％）相比，化合物2和3均通过将Ehrlich腹水癌细胞的活力分别降低至41.0％和42.2％而表现出中等的细胞毒性活性。