Delicate animal experiments and microdose clinical trials using short-lived radioisotopes require rapid preparation with high accuracy and careful attention to safety within a limited timeframe. We have developed an open-style electrowetting on dielectric (EWOD) device containing dimple structures for the rapid preparation of radiolabelled reagents. The device was demonstrated by automatic preparation of a technetium diethylenetriamine pentaacetate (^99mTc-DTPA) with high chelation efficiency (99.7 ± 0.13%). Additionally, we demonstrated the single-photon emission computed tomography/computed tomography imaging of mouse kidney using the ^99mTc-DTPA prepared with the EWOD device. The obtained organ tomographic images were sufficient for the evaluation of mouse models for specific diseases. These results indicate that manual radiolabelling for a small amount of nuclear medicine can be replaced by a process using the proposed EWOD device as a human error reduction technique.

使用短寿命放射性同位素的精细动物实验和微剂量临床试验需要在有限的时间内进行高精度的快速准备并仔细注意安全性。我们开发了一种开放式电介质电润湿 (EWOD) 装置，包含凹坑结构，用于快速制备放射性标记试剂。该装置通过自动制备二亚乙基三胺五乙酸锝 ( ^99m Tc-DTPA) 进行了验证，具有高螯合效率 (99.7 ± 0.13%)。此外，我们还使用 EWOD 设备制备的^99m Tc-DTPA 演示了小鼠肾脏的单光子发射计算机断层扫描/计算机断层扫描成像。获得的器官断层扫描图像足以评估特定疾病的小鼠模型。这些结果表明，少量核医学的手动放射性标记可以被使用所提出的 EWOD 设备作为人为错误减少技术的过程所取代。