Cryopreservation of human peripheral blood mononuclear cells (PBMC) is used in many clinical and research applications to avoid direct and on-site analysis of samples. Storage of PBMC further allows prequalification of donor cells for routine laboratory methods involving the evaluation of immune responses. Previous studies reported changes in cellular composition and phenotype of PBMC following the freezing procedure. In our 12-month follow-up study, we focused on B cells and proportional representation of B cell subpopulations during long-term storage at −80 °C. Over the 12-month period, we observed a gradual decline in B cell viability and recovery. Notably, no changes in the proportional representation of human B cell subpopulations occurred in this period and the functional response elicited by antigen and TLR9 ligand CpG remained comparable to that observed after short-term storage for one month.

人外周血单核细胞 (PBMC) 的冷冻保存用于许多临床和研究应用，以避免对样品进行直接和现场分析。PBMC 的储存进一步允许对涉及免疫反应评估的常规实验室方法的供体细胞进行资格预审。先前的研究报告了冷冻程序后 PBMC 的细胞组成和表型的变化。在我们为期 12 个月的随访研究中，我们专注于 -80 °C 长期储存期间 B 细胞和 B 细胞亚群的比例代表。在 12 个月的时间里，我们观察到 B 细胞活力和恢复逐渐下降。尤其，