Recent studies have revealed the significant role of TEA domain family member 4 (TEAD4) in the development and progression of cancer. However, the potential role of TEAD4 in the progression of bladder cancer (BC) remains to be explored. The aim of this study was to determine whether TEAD4 could serve as a pan-cancer predictor of the prognosis for BC. Based on data mined from public databases, expression levels and clinical value of TEAD4 were identified in BC and human pan-cancers. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis was performed to detect the TEAD4 expression levels in BC cell lines. Gene Set Enrichment Analysis (GSEA) was carried out for functional analysis in BC, and the relationship between infiltrating immune cells and TEAD4 expression was evaluated by the CIBERSORT algorithm in BC and pan-cancer data. TEAD4 was overexpressed and associated with poor prognosis in BC and several types of cancers. GSEA and CIBERSORT algorithm suggested that various pathways including immune-related pathways were enriched in TEAD4 high expression group and several immunocytes infiltrated were correlated with the expression of TEAD4. This study revealed TEAD4 is an immune regulating-related predictor of prognosis for BC and has generalization value in pan-cancer.

中文翻译：

---

TEAD4 是一种免疫调节相关的膀胱癌预后生物标志物，在泛癌中具有推广价值

最近的研究揭示了 TEA 域家族成员 4 ( TEAD4 ) 在癌症的发展和进展中的重要作用。然而，TEAD4在膀胱癌 (BC) 进展中的潜在作用仍有待探索。本研究的目的是确定TEAD4是否可以作为 BC 预后的泛癌预测因子。根据从公共数据库中挖掘的数据，在 BC 和人类泛癌中确定了TEAD4 的表达水平和临床价值。进行逆转录-定量聚合酶链反应 (RT-qPCR) 分析以检测TEAD4BC 细胞系中的表达水平。基因集富集分析（GSEA）在BC中进行功能分析，在BC和泛癌数据中通过CIBERSORT算法评估浸润免疫细胞与TEAD4表达之间的关系。TEAD4过度表达并与 BC 和几种癌症的不良预后相关。GSEA和CIBERSORT算法提示TEAD4高表达组包括免疫相关通路在内的多种通路富集，浸润的多个免疫细胞与TEAD4的表达相关。本研究揭示TEAD4是BC预后的免疫调节相关预测因子，在泛癌中具有推广价值。