Atrial fibrillation (AF), the most familiar heart rhythm disorder, is a major cause of stroke in the world, whereas the mechanism behind AF remains largely unclear. In the current study, we used the RNA-seq method to identify 275 positively regulated mRNAs and 117 negatively regulated mRNAs in AF compared to healthy controls. Through bioinformatic analysis, it indicated that these distinctively expressed genes took part in regulating multiple AF-related biological processes and pathways, such as platelet aggregation, platelet activation, pri-miRNA transcription, and transforming growth factor-beta (TGF-β) receptor signaling pathway. Protein-protein interaction (PPI) network analysis identified ITGB5, SRC, ACTG1, ILK, ITGA2B, ITGB3, TUBB4B, CDK11A, PAFAH1B1, CDK11B, and TUBG1 as hub regulators in AF. Moreover, the quantitative real-time PCR (qRT-PCR) assay was conducted and revealed that these hub genes were remarkably overexpressed in AF samples compared to normal samples. We believed that this study would enrich the understanding of the pathogenesis of AF and enable further research on the pathogenesis of AF.

中文翻译：

---

mRNA调控网络的构建揭示了心房颤动的关键基因

心房纤颤（AF）是最常见的心律失常，是世界上中风的主要原因，而AF背后的机制仍不清楚。在当前的研究中，我们使用RNA-seq方法与健康对照组相比，可以鉴定AF中275个正调控的mRNA和117个负调控的mRNA。通过生物信息学分析表明，这些独特表达的基因参与了调节与AF相关的生物学过程和途径，如血小板聚集，血小板活化，pri-miRNA转录和转化生长因子-β（TGF- β）。）受体信号传导途径。蛋白质相互作用（PPI）网络分析确定ITGB5，SRC，ACTG1，ILK，ITGA2B，ITGB3，TUBB4B，CDK11A，PAFAH1B1，CDK11B和TUBG1是AF中的中枢调节子。此外，进行了定量实时PCR（qRT-PCR）分析，并揭示了与正常样品相比，这些毂基因在AF样品中显着过表达。我们相信这项研究将丰富对房颤的发病机理的了解，并使对房颤的发病机理有进一步的研究。