RNA interference (RNAi) has been proved to be a viable method for agricultural pest control. Due to the limited uptake of dsRNA in hemiptera insects, this study used nanocarrier SPc (star polycation) transdermal delivery systems to deliver two truncated fragments (P1/P2) dsRNA of the CYP6CY3 for silencing this target gene in Aphis gossypii. After the cotton aphid was sprayed with the SPc + dsP1/P2 mixture, the expression level of target gene in SPc + dsP1 treatment group was not different from that in dsP1 group at 24 h, 48 h, and significantly lower than that in dsP1 group at 60 h, 72 h, respectively; and the expression level of target gene in SPc + dsP2 treatment group was not different from that in dsP2 group at 24 h, and significantly lower than that in dsP2 group from 48 h, 60 h, 72 h, respectively. In addition, the expression level was continuously silenced after spraying the SPc + dsP1/P2 mixture and significant reduced by 79.7% and 84.3% at 48 h compared with the H₂O control group, the mortality rate reached 48.09% and 43.18% at 84 h, respectively. And the cumulative reproduction number of cotton aphids also decreased, but the cumulative death number of newborn nymphs had an increase trend, compared with the control groups. Bioassays after RNAi showed that the silencing of CYP6CY3 increased the susceptibility of the 4^th instar aphid to imidacloprid, and increased mortality by 67.21% and 58.69% at 96 h, respectively. The life table parameters of the offspring from the 4^th instar cotton aphids from the SPc + dsP1/P2 treatment groups showed that the offspring had a longer pre-reproductive period and post-reproductive period. The intrinsic growth rate was 0.231 ± 0.005, 0.210 ± 0.013 and the finite growth rate was 1.260 ± 0.007 and 1.234 ± 0.016 in the SPc + dsP1/P2 treatment group, these two parameters of the two groups were lower than that of the corresponding control，the population doubling time of the two groups was prolonged and the developmental duration was delayed. These results indicate that CYP6CY3 plays a key role in the growth, development, reproduction and detoxification ability in cotton aphids, and may be as a potential RNAi target for controlling aphids, laying the foundation for the development of new environmentally-friendly RNA pesticides in this field.

RNA 干扰 (RNAi) 已被证明是一种可行的农业害虫防治方法。由于dsRNA的半翅目中的昆虫的有限的吸收，本研究中使用的纳米载体SPC（星形聚阳离子）的透皮递送系统来递送两种截短片段（P1 / P2）的dsRNA的的CYP6CY3用于沉默本靶基因棉蚜。棉蚜喷洒SPc+dsP1/P2混合液后，SPc+dsP1处理组靶基因表达水平在24h、48h与dsP1组无差异，显着低于dsP1组分别在 60 小时、72 小时；SPc+dsP2治疗组靶基因的表达水平在24h与dsP2组无差异，分别在48h、60h、72h显着低于dsP2组。此外，喷洒SPc + dsP1/P2混合物后表达水平持续沉默，与H ₂相比，48 h时表达水平显着降低79.7%和84.3%。O对照组，84 h死亡率分别达到48.09%和43.18%。与对照组相比，棉蚜的累积繁殖数也有所减少，但新生若虫的累积死亡数有增加的趋势。后的RNAi生物测定表明，沉默CYP6CY3增加了4的易感性^个由67.21％和58.69％，在96小时分别龄蚜虫对吡虫啉，和死亡率增加。从4后代的生命表参数^第SPc+dsP1/P2处理组的幼龄棉蚜表现出后代具有较长的前繁殖期和繁殖后期。SPc+dsP1/P2治疗组内在增长率分别为0.231±0.005、0.210±0.013和有限增长率分别为1.260±0.007和1.234±0.016，两组的这两个参数均低于相应的对照组，两组的种群倍增时间延长，发育持续时间延长。这些结果表明CYP6CY3在棉蚜的生长、发育、繁殖和解毒能力中起关键作用，可能作为防治蚜虫的潜在RNAi靶标，为开发新型环保型RNA农药奠定基础。场地。