Riparian vegetation is constantly subjected to environmental stresses and reactive oxygen species (ROS) are generated in the plant tissue due to the stress. The study assessed the environmental stress in vegetation by quantifying the foliar hydrogen peroxide (H₂O₂) concentration across the elevation gradient of riparian zone of Hii River in Japan. Leaf samples of four common riparian species, Phragmites australis, Phragmites karka, Juglans mandshurica, and Salix pierotii, were used for the investigation. Leaf samples were collected at different elevations along the river. The results indicated that foliar H₂O₂ concentrations of P. australis, P. karka, and S. pierotii decrease with lower elevations indicating low levels of stress due to higher availability of soil moisture. However, J. mandshurica showed the opposite trend. This tree species had a higher concentration of foliar H₂O₂ at lower elevations. J. mandshurica had a higher hydrogen peroxide content with decreasing soil nitrogen concentration, indicating that J. mandshurica prefer fertile soil, especially with regard to the nitrogen content. The results clearly indicate that for J. mandshurica, the preferential location and stress was mainly based on soil nutrient availability. Our findings reveal that hydrogen peroxide is a very efficient and reliable index for quantifying environmental stress for vegetation management.

河岸植被不断受到环境压力，由于压力，植物组织中会产生活性氧（ROS）。该研究通过量化日本Hii河沿岸带海拔梯度上的叶面过氧化氢（H ₂ O ₂）浓度来评估植被中的环境胁迫。研究使用了四种常见河岸种的叶子样本，即芦苇，芦苇，喀尔干，胡桃木和沙柳。沿河的不同海拔收集了叶片样本。结果表明，叶面的H ₂ O ₂浓度为芦苇，P. karka，和S. pierotii减少与由于土壤湿度的更高的可用性低海拔指示应力的低的水平。然而，曼氏草显示出相反的趋势。该树种在较低海拔高度具有较高的叶面H ₂ O ₂浓度。随着土壤氮浓度的降低，曼氏man具有更高的过氧化氢含量，这表明曼氏prefer更喜欢肥沃的土壤，尤其是在氮含量方面。结果清楚地表明，对于J. mandshurica，优选位置和胁迫主要基于土壤养分的可利用性。我们的发现表明，过氧化氢是量化植被管理环境压力的一种非常有效和可靠的指标。