The high cost of commercial cellulases still hampers the economic competitiveness of the production of fuels and chemicals from lignocellulosic biomasses. This cost may be decreased by the on-site production of cellulases with the integrated use of the lignocellulosic biomass as carbon source. This integrated approach was evaluated in the present study whereby steam-pretreated sugarcane bagasse (SPSB) was used as carbon source for the production of cellulases by Trichoderma reesei Rut C30. The use of SPSB resulted in an enzyme preparation with a cellulase activity of 1.93 FPU/mL. Additionally, a significant β-glucosidase activity, of 0.37 BGU/mL, was achieved in buffered media, which is not a common feature of T. reesei culture supernatants, indicating the importance of pH control during enzyme production. The hydrolysis of SPSB with the laboratory-made mixture resulted in a glucose yield of 80%, which was equivalent to those observed for control experiments using commercial enzymes. It was shown that SPSB is a promising carbon source for the production of cellulases and β-glucosidases by T. reesei Rut C30 and that the enzyme preparation obtained is effective for the hydrolysis of SPSB without the need for β-glucosidases supplementation, supporting the on-site integrated approach to decrease the cost of the enzymatic hydrolysis of lignocellulosic biomass.

商业纤维素酶的高成本仍然阻碍了由木质纤维素生物质生产燃料和化学品的经济竞争力。可以通过现场生产纤维素酶和木质纤维素生物质作为碳源的综合使用来降低成本。在本研究中评估了这种综合方法，其中蒸汽预处理的甘蔗渣（SPSB）被用作里氏木霉Rut C30生产纤维素酶的碳源。SPSB的使用产生了一种纤维素酶活性为1.93 FPU / mL的酶制剂。此外，在缓冲液中可达到0.37 BGU / mL的显着β-葡萄糖苷酶活性，这不是里氏木霉的共同特征。培养上清液，表明在酶生产过程中控制pH的重要性。用实验室制得的混合物水解SPSB可以得到80％的葡萄糖收率，这与使用商业酶进行的对照实验所观察到的结果相同。结果表明，SPSB是里氏木霉Rut C30生产纤维素酶和β-葡萄糖苷酶的有前途的碳源，并且所获得的酶制剂可有效地水解SPSB，而无需补充β-葡萄糖苷酶，从而支持现场整合的方法来降低木质纤维素生物质酶促水解的成本。