Background

Oleanolic acid (OA) has notable anti-inflammatory and anti-tumor effects, but the role of OA in cerebral ischemia-reperfusion injury (CIRI) has not been reported so far.

Methods

Oxygen and glucose deprivation/reoxygenation (OGD/R) model was induced in PC12 cells. MTT assay was used to detect the cell viability of PC12 cells, while ELISA assay detected the expression of TNF-α, IL-1β and IL-6. The expression of superoxide dismutase (SOD), malondialdehyde (MDA) and reactive oxygen species (ROS) was detected by the appropriate kits, and cell apoptosis by Tunel technique. Western blot assay detected the expression of apoptosis-related proteins. The cell transfection technique overexpressed miR-142‐5p. After overexpressing miR-142‐5p by cell transfection technique, the expression of miR-142‐5p was detected by RT-qPCR.

Results

Besides the ability to promote cell acitivity, OA ameliorated OGD/R-induced inflammatory response and apoptosis in PC12 cells. Moreover, the capability of OA to alleviate OGD/R-induced inflammation and apoptosis in PC12 cells was observed to be related to the down-regulation of miR-142‐5p.

Conclusion

OA improved inflammatory response and apoptosis of PC12 cells induced by OGD/R through downregulating miR-142‐5P

中文翻译：

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齐墩果酸可通过下调miR-142-5P改善OGD / R诱导的PC12细胞的炎症反应和细胞凋亡。

背景

齐墩果酸（OA）具有显着的抗炎和抗肿瘤作用，但迄今为止尚未报道OA在脑缺血再灌注损伤（CIRI）中的作用。

方法

在PC12细胞中诱导了氧和葡萄糖的剥夺/复氧（OGD / R）模型。MTT法检测PC12细胞的活力，ELISA法检测TNF-α，IL-1β和IL-6的表达。用适当的试剂盒检测超氧化物歧化酶（SOD），丙二醛（MDA）和活性氧（ROS）的表达，并通过Tunel技术检测细胞凋亡。Western blot法检测细胞凋亡相关蛋白的表达。细胞转染技术过表达miR-142-5p。通过细胞转染技术过表达miR-142-5p后，通过RT-qPCR检测miR-142-5p的表达。

结果

除了能够促进细胞活性外，OA还改善了OGD / R诱导的PC12细胞炎症反应和细胞凋亡。此外，观察到OA缓解PC12细胞中OGD / R诱导的炎症和凋亡的能力与miR-142-5p的下调有关。

结论

OA通过下调miR-142-5P来改善OGD / R诱导的PC12细胞的炎症反应和细胞凋亡