ABSTRACT

A pretreatment method for detecting the available (water-soluble) tetracycline (TC) contents in soils was developed using a molecularly imprinted polymer (MIP) synthesized with TC as the template. The infrared spectroscopy, scanning electron microscopy, and TC sorption isotherms revealed a specific TC binding with the synthesized MIP in water. Upon extraction and concentration from water by the MIP, the target TC could be directly analyzed by high-performance liquid chromatography (HPLC). The parameters for the pretreatment method were optimized with TC-spiked water and soils, with observed TC recoveries from water and soils equal to 74.7 ± 4.6% to 95.5 ± 6.5% (n = 3) and 48.1 ± 3.3% to 72.1 ± 2.7% (n = 3), respectively. The detected available TC contents in three test soil samples were in the range of 2.3 ± 0.1 to 3.0 ± 0.1 μg/kg (n = 3), only13.8%-39.4% of the TC contents detected by the conventional solvent-extraction method coupled with a solid-phase extraction (SPE) technique. The observed TC distribution in a vertical soil profile indicated that the proposed method for available antibiotic content coupled with the total extracted antibiotic content via the conventional (organic-solvent) extraction can be utilized to evaluate the available TC level and its mobility in contaminated soils.

摘要

使用以 TC 为模板合成的分子印迹聚合物 (MIP) 开发了一种用于检测土壤中有效（水溶性）四环素 (TC) 含量的预处理方法。红外光谱、扫描电子显微镜和 TC 吸附等温线揭示了与水中合成的 MIP 的特定 TC 结合。通过 MIP 从水中提取和浓缩后，目标 TC 可以通过高效液相色谱 (HPLC) 直接分析。预处理方法的参数用加标 TC 的水和土壤进行了优化，观察到的 TC 从水和土壤中回收率等于 74.7 ± 4.6% 到 95.5 ± 6.5% (n = 3) 和 48.1 ± 3.3% 到 72.1 ± 2.7% （n = 3），分别。三个测试土壤样品中检测到的有效 TC 含量在 2.3 ± 0.1 到 3.0 ± 0.1 μg/kg (n = 3) 的范围内，只有 13。传统溶剂萃取法结合固相萃取 (SPE) 技术检测到的 TC 含量为 8%-39.4%。在垂直土壤剖面中观察到的 TC 分布表明，所提出的有效抗生素含量与通过常规（有机溶剂）萃取的总提取抗生素含量相结合的方法可用于评估可用 TC 水平及其在污染土壤中的迁移率。