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High-throughput sequencing-based analysis of bacterial communities associated with Barbour's seahorses (Hippocampus barbouri) from Surigao del Norte, Philippines
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2021-05-24 , DOI: 10.1111/lam.13511
R C M H Ortega 1 , S R M Tabugo 1 , J G T Martinez 1 , C S Padasas 1 , M P Balolong 2 , J L Balcázar 3, 4
Affiliation  

This study identified the bacterial community composition associated with Barbour's seahorses (Hippocampus barbouri). Seahorses and samples from the surrounding environment (sediment and water) were collected from Cantiasay Island in Surigao del Norte, Philippines. Genomic DNA was isolated from these samples, and the V1–V3 region of the 16S rRNA gene was amplified and sequenced on the Illumina MiSeq platform. There were 929 803 sequence reads corresponding to 859 operational taxonomic units (OTUs), which were obtained from the six genomic libraries. The results showed that the most abundant OTUs were affiliated to the phylum Proteobacteria, particularly those belonging to the genera Shewanella, Pseudomonas, Acinetobacter and Aeromonas, which were found to be dominant in seahorse gut samples. The only exception was for the skin of male seahorses, which was dominated by members belonging to the genus Bacillus (phylum Firmicutes). These findings on bacterial community structure and composition may shed light on therapeutic claims of Barbour's seahorses and help in the conservation of this species.

中文翻译:

基于高通量测序分析与来自菲律宾北苏里高的巴伯海马(Hippocampus barbouri)相关的细菌群落

这项研究确定了与巴伯海马(Hippocampus barbouri)相关的细菌群落组成。来自菲律宾北苏里高的 Cantiasay 岛收集了来自周围环境(沉积物和水)的海马和样本。从这些样本中分离出基因组 DNA,并在 Illumina MiSeq 平台上对 16S rRNA 基因的 V1-V3 区域进行扩增和测序。有 929 803 个序列读数对应于 859 个操作分类单元 (OTU),它们是从六个基因组文库中获得的。结果表明,最丰富的OTUs属于变形菌门,特别是属于希瓦氏菌属、假单胞菌属不动杆菌属的那些。气单胞菌,这被认为是海马肠样品中占主导地位。唯一的例外是雄性海马的皮肤,它被属于芽孢杆菌属厚壁菌门)的成员所支配。这些关于细菌群落结构和组成的发现可能会阐明巴伯海马的治疗主张,并有助于保护该物种。
更新日期:2021-05-24
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