In Europe, swine represent economically important farm animals and furthermore have become a preferred preclinical large animal model for biomedical studies, transplantation and regenerative medicine research. The need for typing of the swine leukocyte antigen (SLA) is increasing with the expanded use of pigs as models for human diseases and organ-transplantation experiments and their use in infection studies and for design of veterinary vaccines. In this study, we characterised the SLA class I (SLA-1, SLA-2, SLA-3) and class II (DRB1, DQB1, DQA) genes of 549 farmed pigs representing nine commercial pig lines by low-resolution (Lr) SLA haplotyping. In total, 50 class I and 37 class II haplotypes were identified in the studied cohort. The most common SLA class I haplotypes Lr-04.0 (SLA-1*04XX-SLA-3*04XX(04:04)-SLA-2*04XX) and Lr-32.0 (SLA-1*07XX-SLA-3*04XX(04:04)-SLA-2*02XX) occurred at frequencies of 11.02 and 8.20% respectively. For SLA class II, the most prevalent haplotypes Lr-0.15b (DRB1*04XX(04:05/04:06)-DQB1*02XX(02:02)-DQA*02XX) and Lr-0.12 (DRB1*06XX-DQB1*07XX-DQA*01XX) occurred at frequencies of 14.37 and 12.46% respectively. Meanwhile, our laboratory has contributed to several vaccine correlation studies (e.g. Porcine Reproductive and Respiratory Syndrome Virus, Classical Swine Fever Virus, Foot-and-Mouth Disease Virus and Swine Influenza A Virus) elucidating the immunodominance in the T-cell response with antigen specificity dependent on certain SLA-I and SLA-II haplotypes. Moreover, these SLA–immune response correlations could facilitate tailored vaccine development, as SLA-I Lr-04.0 and Lr-32.0 as well as SLA-II Lr-0.15b and Lr-0.12 are highly abundant haplotypes in European farmed pigs.

中文翻译：

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欧洲养殖猪猪白细胞抗原基因多样性比较分析

在欧洲，猪是经济上重要的农场动物，而且已成为生物医学研究、移植和再生医学研究的首选临床前大型动物模型。随着猪被广泛用作人类疾病和器官移植实验的模型以及它们在感染研究和兽用疫苗设计中的应用，对猪白细胞抗原 (SLA) 分型的需求正在增加。在这项研究中，我们表征了 SLA I 类（SLA-1、SLA-2、SLA-3）和 II 类（DRB1、DQB1、DQA) 549 头养殖猪的基因，通过低分辨率 (Lr) SLA 单倍型分析代表 9 个商业猪品系。在研究队列中总共鉴定了 50 个 I 类和 37 个 II 类单倍型。最常见的 SLA I 类单倍型 Lr-04.0 (SLA-1*04XX-SLA-3*04XX(04:04)-SLA-2*04XX) 和 Lr-32.0 (SLA-1*07XX-SLA-3*04XX) (04:04)-SLA-2*02XX) 发生的频率分别为 11.02% 和 8.20%。对于 SLA II 类，最普遍的单倍型 Lr-0.15b (DRB1*04XX(04:05/04:06)-DQB1*02XX(02:02)-DQA*02XX) 和 Lr-0.12 (DRB1*06XX-DQB1 *07XX-DQA*01XX) 发生的频率分别为 14.37% 和 12.46%。同时，我们实验室还参与了多项疫苗相关性研究（例如猪繁殖与呼吸综合征病毒、猪瘟病毒、口蹄疫病毒和猪流感 A 病毒）阐明了 T 细胞反应中的免疫优势，抗原特异性取决于某些 SLA-I 和 SLA-II 单倍型。此外，这些 SLA 免疫反应相关性可以促进定制疫苗的开发，因为 SLA-I Lr-04.0 和 Lr-32.0 以及 SLA-II Lr-0.15b 和 Lr-0.12 是欧洲养殖猪中高度丰富的单倍型。