Cytokinins are a class of phytohormones that promote cell division and differentiation and are thought to affect plant immunity to multiple pathogens. However, a comprehensive analysis of cytokinin dehydrogenase/oxidase (CKX) family genes in cabbage has not been reported. In this study, a total of 36 CKX genes were identified using a genome-wide search method. Phylogenetic analysis classified these genes into three groups. They were distributed unevenly across nine chromosomes in B. oleracea, and 15 of them did not contain any introns. The results of colinearity analysis showed that 36 CKX gene in Arabidopsis was present in several copies in the Brassica oleracea genome. An analysis of cis-acting elements indicated that all genes possessed at least one stress or hormone responsive cis-acting element. A heatmap of CKX gene expression showed the patterns of expression of these genes in various tissues and organs. Three genes (Bol028363, Bol031036 and Bol018140) were relatively highly expressed in all of the investigated tissues under normal conditions, showing the expression profile of housekeeping genes. Generally, the expression patterns of CKX genes in Jingfeng 1 and Xiangan 336 were quite different under the same treatment. Notably, three genes (Bol020547, Bol028392 and Bol045724) were significantly down-regulated and up-regulated in the susceptible and resistant material, respectively, after inoculation, which may indicate their crucial roles in resistance to clubroot disease. The results provide insights for better understanding the roles of CKX genes in the B. oleracea–P. brassicae interaction.

细胞分裂素是一类促进细胞分裂和分化的植物激素，被认为会影响植物对多种病原体的免疫力。然而，尚未报道对卷心菜中细胞分裂素脱氢酶/氧化酶（CKX）家族基因的综合分析。在这项研究中，使用全基因组搜索方法共鉴定了 36 个CKX基因。系统发育分析将这些基因分为三组。它们在甘蓝中的9 条染色体上分布不均，其中 15 条不含任何内含子。共线性分析结果表明，拟南芥中的36个CKX基因在甘蓝中存在多个拷贝。基因组。顺式作用元件的分析表明所有基因都具有至少一种应激或激素反应性顺式作用元件。CKX基因表达的热图显示了这些基因在各种组织和器官中的表达模式。三个基因（Bol028363、Bol031036和Bol018140）在正常条件下在所有研究的组织中相对高表达，显示了管家基因的表达谱。一般来说，相同处理下，京丰1号和翔安336中CKX基因的表达模式存在较大差异。值得注意的是，三个基因（Bol020547、Bol028392 和 Bol045724) 在易感和抗性材料中分别显着下调和上调，在接种后，这可能表明它们在抗根肿病中的关键作用。结果为更好地理解CKX基因在B. oleracea - P. brasicae相互作用中的作用提供了见解。