Purpose

Malaria and toxoplasmosis are important public health diseases affecting millions of people and animals each year, and there is a continuing need for new and improved treatments for them. Plants have provided many opportunities for new drug leads in pharmacology.

Methods

We examined 43 crude extracts from Mongolian plants for their activities against the Plasmodium falciparum 3D7 strain and the Toxoplasma gondii RH strain using a SYBR Green-based fluorescence assay and a fluorescence-based assay, respectively. The potential toxicity of these extracts was also assessed on human foreskin fibroblast cells (HFF) using a cell viability assay.

Results

From the initial screenings, 11 and 7 crude extracts were effective against T. gondii and P. falciparum, respectively, at 100 µg/ml concentration (≥ 80% inhibition activity). The 50% cytotoxic concentrations of the extracts were estimated on HFF cells, and their 50% inhibitory concentrations (IC₅₀s) were calculated. According to our lead criteria (selective index, SI; value ≥ 10), six plants (Galatella dahurica leaf + flower, Leonurus deminutus leaf + flower, Oxytropis trichophysa aerial part, Schultzia crinita whole plant, Leontopodium campestre root, Spirea salicifolia aerial part) inhibited P. falciparum growth at IC₅₀ values of 5.99–64.15 µg/ml (SI values: 10.11–17.02). Amaranthus retroflexus root was highly active against T. gondii (IC₅₀, 19.89 µg/ml; SI value, 38).

Conclusion

This first observation of the anti-Plasmodium and anti-Toxoplasma activities of Mongolian plant extracts shows them to be interesting potential candidates for drug discovery.

中文翻译：

---

体外强效抗疟原虫和抗弓形虫蒙古植物提取物

目的

疟疾和弓形虫病是每年影响数百万人和动物的重要公共卫生疾病，并且持续需要新的和改进的治疗方法。植物为药理学中的新药先导提供了许多机会。

方法

我们分别使用基于 SYBR Green 的荧光测定和基于荧光的测定检测了 43 种蒙古植物粗提物对恶性疟原虫3D7 菌株和弓形虫RH 菌株的活性。还使用细胞活力测定对人包皮成纤维细胞 (HFF) 评估了这些提取物的潜在毒性。

结果

从最初的筛选来看，11 和 7 种粗提物分别在 100 µg/ml 浓度下对弓形虫和恶性疟原虫有效（抑制活性≥80%）。在 HFF 细胞上估计提取物的 50% 细胞毒性浓度，并计算它们的 50% 抑制浓度 (IC ₅₀ s)。根据我们的引线标准（选择性指数，SI;值≥10），六种植物（乳菀属白芷叶+花，益母草deminutus叶+花，棘trichophysa地上部分，Schultzia crinita全株，火绒坎佩斯特里根，绣线菊柳叶地上部分）抑制恶性疟原虫在 IC ₅₀值为 5.99–64.15 µg/ml 时生长（SI 值：10.11–17.02）。反枝苋根兑高活性弓形虫（IC ₅₀，19.89微克/毫升; SI值，38）。

结论

对蒙古植物提取物的抗疟原虫和抗弓形虫活性的首次观察表明，它们是潜在的药物发现候选物。