Canine lymphoma is the most common haematological malignancy in dogs and is typically treated with multidrug chemotherapy. Most cases are at risk of relapse after several courses of chemotherapy and the oncogenic mechanism remains unknown. This study was aimed at identifying genes expressed in canine lymphoma by cDNA microarray. We found elevated expression of Dishevelled, EGL-10 and pleckstrin (DEP) domain-containing 1B (DEPDC1B) in canine lymphoma cells compared with cells and tissues from healthy dogs. Canine DEPDC1B protein was detected in 13 of 41 lymphoma specimens by immunohistochemistry, but was not detected in lymph nodes from normal dogs. Immunoreactive DEPDC1B protein was also detected in several other types of canine tumour. This is the first report documenting the association of DEPDC1B with canine cancer and the results suggest that DEPDC1B might serve as a potential marker or therapeutic target for canine malignancies.

犬淋巴瘤是犬最常见的血液系统恶性肿瘤，通常采用多药化疗进行治疗。大多数病例在几个疗程的化疗后有复发的风险，致癌机制尚不清楚。本研究旨在通过 cDNA 微阵列鉴定在犬淋巴瘤中表达的基因。我们发现 Dishevelled、EGL-10 和含有pleckstrin (DEP) 结构域的 1B ( DEPDC1B) 犬淋巴瘤细胞与健康犬的细胞和组织相比。通过免疫组织化学在 41 份淋巴瘤标本中的 13 份中检测到犬 DEPDC1B 蛋白，但在正常犬的淋巴结中未检测到。在其他几种犬肿瘤中也检测到免疫反应性 DEPDC1B 蛋白。这是第一份记录 DEPDC1B 与犬癌症关联的报告，结果表明 DEPDC1B 可能作为犬恶性肿瘤的潜在标志物或治疗靶点。