Fusarium oxysporum is one of the most common soil borne pathogens over the world. This work tried to introduce safe and efficient agent in controlling this pathogen infection in cucumber plants, beside that understanding the mechanism at which fabitine enables the cucumber plants to fight wilt disease. Also, this works introduce a molecular modeling for the direct interaction between fabatin and the Fusarium oxysporum. We evaluated the impact of defensin isolated from broad bean debris (fabatin) against the Fusarium wilt disease in the greenhouse, followed by the analysis of defense-related enzymes, including superoxide dismutase, polyphenol oxidase, soluble peroxidase, cell wall-bound peroxidase, catalase, lactate dehydrogenase, phenylalanine ammonia-lyase, chitinase, and glucanase. Moreover, the growth-regulating protein of the fungus (Snt2, a BAH/PHD) was docked with the fabatin. Fabatin reduced the disease severity from 18.52 to 0.0% by 100% disease reduction, 30 days after transplanting, and from 66.67 to 33.33% by 50.01% disease reduction after 60 days. Interestingly fabatin spray induced upregulation of the studied defense enzymes. The sprayed group showed significant continuous increase in defense related enzymes under infection. Furthermore, fabatin showed a high binding affinity to the Bromo-adjacent domain (BAH) of the Snt2 by - 214.42 kacl/mol and this can prevent the transcriptional regulation processes of the fungus. The present study proved that the exogenous spray fabatin is an effective way in controlling cucumber Fusarium wilt. This is achieved through the enhancement the defense related enzymes which regulate the resistance mechanism in the plants. Moreover, fabatin can directly influence the fungal growth.

尖孢镰刀菌是世界上最常见的土壤传播病原体之一。这项工作试图引入安全有效的试剂来控制黄瓜植株中的这种病原体感染，此外还要了解法比汀使黄瓜植株对抗枯萎病的机理。此外，这项工作介绍了 fabatin 和尖孢镰刀菌之间直接相互作用的分子模型. 我们评估了从蚕豆碎片 (fabatin) 中分离的防御素对温室中枯萎病的影响，然后分析了与防御相关的酶，包括超氧化物歧化酶、多酚氧化酶、可溶性过氧化物酶、细胞壁结合过氧化物酶、过氧化氢酶、乳酸脱氢酶、苯丙氨酸解氨酶、几丁质酶和葡聚糖酶。此外，真菌的生长调节蛋白（Snt2，一种 BAH/PHD）与 fabatin 对接。Fabatin 在移植后 30 天将疾病严重程度从 18.52% 降低到 0.0%，疾病减少 100%，60 天后疾病严重程度从 66.67% 减少到 33.33%，疾病减少 50.01%。有趣的是，fabatin 喷雾诱导了所研究的防御酶的上调。喷洒组在感染下防御相关酶显着持续增加。此外，fabatin 对 Snt2 的溴相邻结构域 (BAH) 显示出高结合亲和力 - 214.42 kacl/mol，这可以阻止真菌的转录调节过程。本研究证明外源喷雾法巴丁是防治黄瓜枯萎病的有效方法。这可以通过增强防御相关的酶来实现，这些酶调节植物的抗性机制。此外，Fabatin可以直接影响真菌的生长。这是通过增强调节植物抗性机制的防御相关酶来实现的。此外，fabatin 可以直接影响真菌的生长。这可以通过增强防御相关的酶来实现，这些酶调节植物的抗性机制。此外，fabatin 可以直接影响真菌的生长。