Significance: Intraoperative parameters of renal cortical microperfusion (RCM) have been associated with postoperative ischemia/reperfusion injury. Laser speckle contrast imaging (LSCI) could provide valuable information in this regard with the advantage over the current standard of care of being a non-contact and full-field imaging technique. Aim: Our study aims to validate the use of LSCI for the visualization of RCM on ex vivo perfused human-sized porcine kidneys in various models of hemodynamic changes. Approach: A comparison was made between three renal perfusion measures: LSCI, the total arterial renal blood flow (RBF), and sidestream dark-field (SDF) imaging in different settings of ischemia/reperfusion. Results: LSCI showed a good correlation with RBF for the reperfusion experiment (0.94 ± 0.02; p < 0.0001) and short- and long-lasting local ischemia (0.90 ± 0.03; p < 0.0001 and 0.81 ± 0.08; p < 0.0001, respectively). The correlation decreased for low flow situations due to RBF redistribution. The correlation between LSCI and SDF (0.81 ± 0.10; p < 0.0001) showed superiority over RBF (0.54 ± 0.22; p < 0.0001). Conclusions: LSCI is capable of imaging RCM with high spatial and temporal resolutions. It can instantaneously detect local perfusion deficits, which is not possible with the current standard of care. Further development of LSCI in transplant surgery could help with clinical decision making.

中文翻译：

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使用激光散斑对比成像实时可视化肾脏微灌注

意义：肾皮质微灌注 (RCM) 的术中参数与术后缺血/再灌注损伤有关。激光散斑对比成像 (LSCI) 可以在这方面提供有价值的信息，其优势在于作为非接触式和全视野成像技术的当前护理标准。目的：我们的研究旨在验证 LSCI 在各种血流动力学变化模型中体外灌注人大小猪肾上 RCM 的可视化的使用。方法：对三种肾脏灌注测量进行了比较：LSCI、肾动脉总血流量 (RBF) 和不同缺血/再灌注环境下的侧流暗场 (SDF) 成像。结果：LSCI 在再灌注实验中显示出与 RBF 的良好相关性（0.94 ± 0.02；p < 0。0001) 和短期和长期局部缺血 (0.90 ± 0.03; p < 0.0001 和 0.81 ± 0.08; p < 0.0001, 分别)。由于 RBF 重新分配，低流量情况下的相关性降低。LSCI 和 SDF 之间的相关性 (0.81 ± 0.10; p < 0.0001) 显示优于 RBF (0.54 ± 0.22; p < 0.0001)。结论：LSCI 能够以高空间和时间分辨率对 RCM 成像。它可以即时检测局部灌注不足，这在当前的护理标准中是不可能的。LSCI 在移植手术中的进一步发展有助于临床决策。0001) 表现出优于 RBF (0.54 ± 0.22; p < 0.0001)。结论：LSCI 能够以高空间和时间分辨率对 RCM 成像。它可以即时检测局部灌注不足，这在当前的护理标准中是不可能的。LSCI 在移植手术中的进一步发展有助于临床决策。0001) 表现出优于 RBF (0.54 ± 0.22; p < 0.0001)。结论：LSCI 能够以高空间和时间分辨率对 RCM 成像。它可以即时检测局部灌注不足，这在当前的护理标准中是不可能的。LSCI 在移植手术中的进一步发展有助于临床决策。