Dialdehyde hydroxypropyl methylcellulose (DHPMC)/Collagen (DHPMC/COL) solutions were prepared with different DHPMC contents (0–70 wt%). According to the viscosimetric method, all samples were compatible. The compatibility was related to Schiff’s base, which formed between the amino groups of collagen and the dialdehyde groups of DHPMC. When DHPMC content increased, the cross-linking degree increased, and the synchronous negative cross-peaks between the C-O(H) of DHPMC at 1031 cm⁻¹ and amide bands of collagen at 1623 cm⁻¹, 1523 cm⁻¹, and 1238 cm⁻¹ indicated that hydrogen bonds mainly formed between DHPMC and collagen. As a result, the thermal stability improved. The morphology showed more sheet-like structures than collagen. When the DHPMC content was 50 wt%, the thermal denaturation temperature (Td) and residual weight% were 8.5 °C and 10.22%, respectively, higher than other samples. However, the cross-linking degree decreased as the DHPMC content increased further. The intensity of negative cross-peak was decreased in synchronous correlation spectra, while the intensity of cross-peak at 1031 cm⁻¹ was enhanced, indicating that hydrogen bonds formed between the C–O and C–O(H) of the DHPMC were enhanced. Consequently, the thermal stability decreased and more porous structures were observed compared to those when DHPMC content was 50 wt%.

Graphic abstract

中文翻译：

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二醛羟丙基甲基纤维素/胶原蛋白（DHPMC / COL）溶液的制备和表征

制备了具有不同DHPMC含量（0–70 wt％）的二醛羟丙基甲基纤维素（DHPMC）/胶原蛋白（DHPMC / COL）溶液。根据粘度法，所有样品都是相容的。相容性与席夫碱有关，席夫碱形成于胶原蛋白的氨基和DHPMC的二醛基之间。当DHPMC含量增加，交-联度增加，并且DHPMC的CO（H）之间的同步负交叉峰在1031厘米^-1和胶原的酰胺频带在1623厘米^-1，1523厘米^-1，和1238 cm ^-1表示氢键主要在DHPMC和胶原之间形成。结果，提高了热稳定性。形态显示出比胶原更多的片状结构。当DHPMC含量为50wt％时，热变性温度（Td）和残余重量％分别比其他样品高8.5℃和10.22％。但是，随着DHPMC含量的进一步增加，交联度降低。在同步相关光谱中，负交叉峰的强度减小，而在1031 cm ^-1处的交叉峰的强度增强，表明DHPMC的C–O和C–O（H）之间形成的氢键为增强。因此，与DHPMC含量为50重量％时相比，热稳定性降低并且观察到更多的多孔结构。

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