QUAREP-LiMi: a community endeavor to advance quality assessment and reproducibility in light microscopy,Nature Methods

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QUAREP-LiMi: a community endeavor to advance quality assessment and reproducibility in light microscopy
Nature Methods ( IF 36.1 ) Pub Date : 2021-05-21 , DOI: 10.1038/s41592-021-01162-y
Ulrike Boehm ₁ , Glyn Nelson ₂ , Claire M Brown ₃ , Steve Bagley ₄ , Peter Bajcsy ₅ , Johanna Bischof ₆ , Aurelien Dauphin ₇ , Ian M Dobbie ₈ , John E Eriksson ₉ , Orestis Faklaris ₁₀ , Julia Fernandez-Rodriguez ₁₁ , Alexia Ferrand ₁₂ , Laurent Gelman ₁₃ , Ali Gheisari ₁₄ , Hella Hartmann ₁₄ , Christian Kukat ₁₅ , Alex Laude ₂ , Miso Mitkovski ₁₆ , Sebastian Munck _{17,

18,

19} , Alison J North ₂₀ , Tobias M Rasse ₂₁ , Ute Resch-Genger ₂₂ , Lucas C Schuetz ₂₃ , Arne Seitz ₂₄ , Caterina Strambio-De-Castillia ₂₅ , Jason R Swedlow ₂₆ , Roland Nitschke _{27,

28}

Affiliation

Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Bioimaging Unit, Newcastle University, Newcastle upon Tyne, UK.
Advanced BioImaging Facility (ABIF), McGill University, Montreal, Quebec, Canada.
Visualisation, Irradiation & Analysis, Cancer Research UK Manchester Institute, Alderley Park, Macclesfield, UK.
National Institute of Standards and Technology, Gaithersburg, MD, USA.
Euro-BioImaging ERIC, Heidelberg, Germany.
Unite Génétique et Biologie du Développement U934, PICT-IBiSA, Institut Curie/Inserm/CNRS/PSL Research University, Paris, France.
Department of Biochemistry, University of Oxford, Oxford, UK.
Turku Bioscience Centre, Euro-Bioimaging ERIC, Turku, Finland.
BCM, University of Montpellier, CNRS, INSERM, Montpellier, France.
Centre for Cellular Imaging, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Imaging Core Facility Biozentrum, University of Basel, Basel, Switzerland.
Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
Light Microscopy Facility, CMCB Technology Platform, TU Dresden, Dresden, Germany.
FACS & Imaging Core Facility, Max Planck Institute for Biology of Ageing, Cologne, Germany.
Light Microscopy Facility, Max Planck Institute of Experimental Medicine, Goettingen, Germany.
VIB BioImaging Core, Leuven, Flanders, Belgium.
VIB-KU Leuven Center for Brain and Disease, Leuven, Flanders, Belgium.
KU Leuven Department for Neuroscience, Leuven, Flanders, Belgium.
The Rockefeller University, New York, NY, USA.
Scientific Service Group Microscopy, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany.
Division Biophotonics, Federal Institute for Materials Research and Testing (BAM), Berlin, Germany.
European Molecular Biology Laboratory, Advanced Light Microscopy Facility, Heidelberg, Germany.
Faculty of Life Sciences, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland.
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA.
Divisions of Computational Biology and Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee, UK.
Life Imaging Center, Albert-Ludwigs University, Freiburg, Germany.
Life Imaging Center and Signalling Research Centres CIBSS and BIOSS, University of Freiburg, Freiburg, Germany.

The community-driven initiative Quality Assessment and Reproducibility for Instruments & Images in Light Microscopy (QUAREP-LiMi) wants to improve reproducibility for light microscopy image data through quality control (QC) management of instruments and images. It aims for a common set of QC guidelines for hardware calibration and image acquisition, management and analysis.

中文翻译：

QUAREP-LiMi：一个致力于推进光学显微镜质量评估和再现性的社区

由社区推动的光学显微镜仪器和图像质量评估和再现性计划 (QUAREP-LiMi) 希望通过仪器和图像的质量控制 (QC) 管理来提高光学显微镜图像数据的再现性。它旨在为硬件校准和图像采集、管理和分析制定一套通用的质量控制指南。

更新日期：2021-05-22

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