The present study was conducted to evaluate the effect of a live culture of Aspergillus oryzae (A; CCT4359) and fibrolytic enzyme (E; Fibrozyme Alltech Inc.) on fibre digestibility by a gas production bioassay and in vitro degradation of maize silage and sugarcane silage. A completely randomized design trial was performed to evaluate: A doses (0, 20, 60 and 100 mg/l), E doses (0, 160, 320 and 480 mg/l) and roughage source (R; maize and sugarcane silage) in a 4 × 4 × 2 factorial arrangement. The inclusion of increasing doses of A and E increased dry matter and neutral detergent fibre in vitro digestibility linearly, but for E this effect occurred only in maize silage. There was a linear increase in the potential for gas production at the highest dose of A only in sugarcane silage, with no effect on lag time (L). Increasing doses of E increased the volume of gases produced linearly, and a trend of linear reduction of L, regardless of the roughage. There was a linear reduction in ammonia-nitrogen concentration in response to increasing doses of A and E, and an increase in acetic acid concentration at the highest dose of A, regardless of roughage. The additives had no synergistic effect on gas production and digestibility, but were efficient in altering the fermentative pattern, demonstrating the potential to increase fibre degradation.

中文翻译：

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米曲霉和纤溶酶复合处理对玉米青贮和甘蔗青贮体外降解、产气和发酵特性的影响

本研究旨在评估活体培养的效果米曲霉(A; CCT4359) 和纤溶酶 (E; Fibrozyme Alltech Inc.) 通过产气生物测定对纤维消化率的影响和体外玉米青贮和甘蔗青贮的降解。进行了一项完全随机的设计试验以评估：A 剂量（0、20、60 和 100 mg/l）、E 剂量（0、160、320 和 480 mg/l）和粗饲料来源（R；玉米和甘蔗青贮饲料）在 4 × 4 × 2 阶乘排列中。包含增加剂量的 A 和 E 增加了干物质和中性洗涤纤维体外消化率呈线性关系，但对于 E，这种影响仅发生在玉米青贮饲料中。仅在甘蔗青贮饲料中，最高剂量的 A 的产气潜力呈线性增加，对时差(L)。无论粗饲料如何，增加 E 的剂量都会线性增加产生的气体量，并且 L 呈线性减少的趋势。无论粗饲料如何，氨氮浓度随着 A 和 E 剂量的增加而线性降低，并且在 A 的最高剂量下乙酸浓度增加。这些添加剂对产气和消化率没有协同作用，但在改变发酵模式方面是有效的，证明了增加纤维降解的潜力。