The major histocompatibility complex (MHC) plays a role in immune response. Among other activities, the bovine MHC genes (BoLA) trigger immune responses, including the activation of antibody-producing B-cells. In this study, White Fulani (n = 24), Red Bororo (n = 5) and Holstein-White × Fulani-cross (n = 11) cattle from Nigeria, and New Zealand Holstein-Friesian × Jersey-cross (n = 40) cattle were used to investigate variability in exon 2 of BoLA-DQA1. Ten alleles were identified using a PCR-Single Strand Conformation Polymorphism (SSCP) approach and their nucleotide sequences confirmed by DNA sequencing. A total of 12.60 % of all nucleotide positions analysed were revealed to be variable and two novel BoLA-DQA1 alleles are reported here for the first time.

主要组织相容性复合体 (MHC) 在免疫反应中发挥作用。在其他活动中，牛 MHC 基因 (BoLA) 会触发免疫反应，包括激活产生抗体的 B 细胞。在本研究中，来自尼日利亚的 White Fulani (n = 24)、Red Bororo (n = 5) 和 Holstein-White × Fulani-cross (n = 11)，以及新西兰的 Holstein-Friesian × Jersey-cross (n = 40) ) 牛被用来研究 BoLA- DQA1外显子 2 的变异性。使用 PCR-单链构象多态性 (SSCP) 方法鉴定了 10 个等位基因，并通过 DNA 测序确认了它们的核苷酸序列。分析的所有核苷酸位置中总共有 12.60% 是可变的，这里首次报道了两个新的 BoLA- DQA1等位基因。