– Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense (Foc), is one of the most destructive diseases affecting banana crops worldwide. Therefore, the development of resistant cultivars is a promising alternative to mitigate the effects of the disease on banana plantations. The objectives of this study were to induce somaclonal variation in banana cultivars of the Silk and Cavendish types and to select somaclones resistant to subtropical race 4, thereby enabling the production of fruit in areas where this race is present. Shoot clump apexes of the Grand Naine and Maçã (Silk) cultivars were grown in MS medium. The cultures were subcultured four times. They were then challenged with fusaric acid (FA) in an experiment consisting of four treatments with different concentrations (0.1, 0.2, 0.3, and 0.4 mM) and five repetitions, each consisting of a Petri dish containing seven multiple shoot clumps in MS culture medium supplemented with 2.5 mg/L benzylamine purine. Multiple shoot clumps without the addition of FA were also used in the experiment, and were subcultured three times and maintained in a dark room. The multiple shoot clumps that survived the treatment with FA were transferred to MS medium and maintained in the growth chamber in the presence of light. The regenerated plants were later planted in tanks containing soil infested with an isolate classified as Foc subtropical race 4 (Foc STR4), and were evaluated for resistance to the pathogen at 90 days after inoculation (d.a.i.). Pathogen structures were confirmed by root clarification and root staining technique. All somaclones of the Maçã (Silk) cultivar were susceptible to Fusarium wilt and two somaclones of the Grand Naine cultivar were selected as resistant. The addition of FA as a selective agent was effective in the selection of somaclones among plants of the Grand Naine cultivar, as shown by the selection of two somaclones resistant to Foc STR4. The next step will consist of the agronomic and market potential validation of the selected somaclones, aiming to confirm their potential use by producers.

– 镰刀菌枯萎病，由尖镰孢f. 引起。 sp。 cubense (Foc) 是影响全世界香蕉作物的最具破坏性的病害之一。因此，开发抗性品种是减轻该病对香蕉种植园影响的一个有前途的替代方案。本研究的目的是诱导 Silk 型和 Cavendish 型香蕉品种的体细胞无性系变异，并选择对亚热带品种 4 具有抗性的体细胞无性系，从而能够在该品种存在的地区生产水果。 Grand Naine 和 Maçã（Silk）品种的枝条顶端在 MS 培养基中生长。将培养物传代培养四次。然后，他们在一项实验中接受镰刀菌酸 (FA) 的挑战，该实验包括四种不同浓度（0.1、0.2、0.3 和 0.4 mM）的处理和五次重复，每次重复由一个培养皿组成，该培养皿在 MS 培养基中含有七个多重芽丛补充2.5mg/L苯甲胺嘌呤。实验中还使用了未添加FA的多个芽丛，并传代培养3次并保存在暗室中。将经 FA 处理后存活的多个芽丛转移至 MS 培养基中，并在有光的情况下维持在生长室中。随后将再生的植物种植在含有被分类为 Foc 亚热带小种 4 (Foc STR4) 的分离株侵染的土壤的水箱中，并在接种后 90 天 (dai) 评估对病原体的抗性。通过根澄清和根染色技术确认病原体结构。 Maçã（丝绸）品种的所有体细胞无性系都对镰刀菌枯萎病敏感，并且选择了 Grand Naine 品种的两个体细胞无性系具有抗性。 添加 FA 作为选择剂对于 Grand Naine 品种植物中体细胞无性系的选择是有效的，如选择两个对 Foc STR4 具有抗性的体细胞无性系所示。下一步将包括对所选体细胞克隆的农艺和市场潜力验证，旨在确认生产者的潜在用途。