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Chilled storage of Asian seabass, Lates calcarifer Bloch semen: Effects of ions, extenders and storage periods on sperm quality and fertilization ability
Journal of Applied Ichthyology ( IF 0.7 ) Pub Date : 2021-05-21 , DOI: 10.1111/jai.14218
Verapong Vuthiphandchai 1 , Traimat Boonthai 2 , Subuntith Nimrat 3
Affiliation  

The development of a chilled storage protocol of fish sperm requires an understanding of sperm biology and function as the activation/inhibition of fish sperm is greatly affected by several environmental factors. This study aimed to determine the effects of ionic and non-ionic solutions, extender types (Ringer's solution, Ca-F HBSS solution, HBSS solution, He and Wood solution, Saline solution, and Modified Cortland solution), and chilled-storage period on sperm quality and fertilization ability of Asian seabass, Lates calcarifer semen. Regulation of Asian seabass sperm motility was dependent on the osmolality of both ionic and non-ionic activation media. The threshold levels on the initiation of sperm motility were detected in KCl (>100 mM), NaCl (>50 mM), CaCl2 (>50 mM), glucose (>300 mM), and mannitol (>100 mM) solutions. Relatively high percentages of sperm motility (>80%) were observed when activated with KCl, NaCl, CaCl2, glucose, and mannitol solutions at above 700, 600, 350, 1,000, and 1,000 mM, respectively. Ringer's solution was the most optimal extender for chilled storage of Asian seabass semen at 2–4°C supported by the retention of sperm motility and viability for 6 days. Semen diluted in Ringer's solution and chilled-stored for 2 days exhibited acceptable fertilization (66.1% ± 6.2%) and hatching (56.4% ± 2.9%) rates. This report, for the first time, describes the ionicity and non-ionicity effects on the motility of Asian seabass sperm.

中文翻译:

亚洲鲈鱼、Lates calcarifer Bloch 精液的冷冻储存:离子、稀释剂和储存期对精子质量和受精能力的影响

鱼精子冷冻储存方案的开发需要了解精子生物学和功能,因为鱼精子的激活/抑制受多种环境因素的影响很大。本研究旨在确定离子和非离子溶液、增量剂类型(林格氏溶液、Ca-F HBSS 溶液、HBSS 溶液、He 和 Wood 溶液、盐水和改良 Cortland 溶液)和冷冻储存期对亚洲鲈鱼,Lates calcarifer精液的精子质量和受精能力。亚洲鲈鱼精子活力的调节取决于离子和非离子激活介质的渗透压。在 KCl (>100 mM)、NaCl (>50 mM)、CaCl 2中检测到精子活力开始的阈值水平(>50 mM)、葡萄糖 (>300 mM) 和甘露醇 (>100 mM) 溶液。当分别用高于 700、600、350、1,000 和 1,000 mM 的KCl、NaCl、CaCl 2、葡萄糖和甘露醇溶液激活时,观察到相对较高百分比的精子活力 (>80%) 。林格氏溶液是亚洲鲈鱼精液在 2–4°C 下冷藏储存的最佳稀释剂,可将精子活力和活力保留 6 天。在林格氏液中稀释并冷藏 2 天的精液表现出可接受的受精率 (66.1% ± 6.2%) 和孵化率 (56.4% ± 2.9%)。该报告首次描述了离子性和非离子性对亚洲鲈鱼精子活力的影响。
更新日期:2021-07-23
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