The development of a chilled storage protocol of fish sperm requires an understanding of sperm biology and function as the activation/inhibition of fish sperm is greatly affected by several environmental factors. This study aimed to determine the effects of ionic and non-ionic solutions, extender types (Ringer's solution, Ca-F HBSS solution, HBSS solution, He and Wood solution, Saline solution, and Modified Cortland solution), and chilled-storage period on sperm quality and fertilization ability of Asian seabass, Lates calcarifer semen. Regulation of Asian seabass sperm motility was dependent on the osmolality of both ionic and non-ionic activation media. The threshold levels on the initiation of sperm motility were detected in KCl (>100 mM), NaCl (>50 mM), CaCl₂ (>50 mM), glucose (>300 mM), and mannitol (>100 mM) solutions. Relatively high percentages of sperm motility (>80%) were observed when activated with KCl, NaCl, CaCl₂, glucose, and mannitol solutions at above 700, 600, 350, 1,000, and 1,000 mM, respectively. Ringer's solution was the most optimal extender for chilled storage of Asian seabass semen at 2–4°C supported by the retention of sperm motility and viability for 6 days. Semen diluted in Ringer's solution and chilled-stored for 2 days exhibited acceptable fertilization (66.1% ± 6.2%) and hatching (56.4% ± 2.9%) rates. This report, for the first time, describes the ionicity and non-ionicity effects on the motility of Asian seabass sperm.

中文翻译：

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亚洲鲈鱼、Lates calcarifer Bloch 精液的冷冻储存：离子、稀释剂和储存期对精子质量和受精能力的影响

鱼精子冷冻储存方案的开发需要了解精子生物学和功能，因为鱼精子的激活/抑制受多种环境因素的影响很大。本研究旨在确定离子和非离子溶液、增量剂类型（林格氏溶液、Ca-F HBSS 溶液、HBSS 溶液、He 和 Wood 溶液、盐水和改良 Cortland 溶液）和冷冻储存期对亚洲鲈鱼，Lates calcarifer精液的精子质量和受精能力。亚洲鲈鱼精子活力的调节取决于离子和非离子激活介质的渗透压。在 KCl (>100 mM)、NaCl (>50 mM)、CaCl ₂中检测到精子活力开始的阈值水平(>50 mM)、葡萄糖 (>300 mM) 和甘露醇 (>100 mM) 溶液。当分别用高于 700、600、350、1,000 和 1,000 mM 的KCl、NaCl、CaCl ₂、葡萄糖和甘露醇溶液激活时，观察到相对较高百分比的精子活力 (>80%) 。林格氏溶液是亚洲鲈鱼精液在 2–4°C 下冷藏储存的最佳稀释剂，可将精子活力和活力保留 6 天。在林格氏液中稀释并冷藏 2 天的精液表现出可接受的受精率 (66.1% ± 6.2%) 和孵化率 (56.4% ± 2.9%)。该报告首次描述了离子性和非离子性对亚洲鲈鱼精子活力的影响。