Journal of Natural Medicines ( IF 2.5 ) Pub Date : 2021-05-21 , DOI: 10.1007/s11418-021-01530-0 Zhao Zhuoyue 1 , Wijitrapha Ruangaram 1 , Eisuke Kato 2
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Acacia concinna (Willd.) DC. is a medicinal plant sourced mainly from Southeast Asia. The pods of Acacia concinna (A. concinna) are a potential candidate to treat or prevent obesity; however, these medicinal attributes have not been examined in detail. In this study, the anti-obesogenic compounds in A. concinna pods were investigated. Chromatographic separation of the pod extract guided by pancreatic lipase inhibitory activity led to the isolation of saponins. Decomposition analysis of the saponins revealed their chemical composition to be acacic acid, monoterpenes, and five types of sugars (glucose, xylose, rhamnose, quinovose, arabinose). The predicted structures of the saponins from decomposition analysis were confirmed by LC–MS analysis, showing that these saponins are mixture of various derivatives of monoterpenes and sugar units. These saponins inhibited pancreatic lipase activity strongly with an IC50 of 7.9 μg/mL, and reduced lipid accumulation in 3T3-L1 adipocytes at 6.3 μg/mL. The saponins also enhanced lipolysis of 3T3-L1 adipocytes at 3.1 or 6.3 μg/mL by mediating the activity of protein kinase A and extracellular signal-regulated kinase pathways, suggesting that this mechanism is partly responsible for the observed reduction of lipid content in adipocytes. The results underline A. concinna as a potential source of the anti-obesogenic candidates for the future treatment and prevention of obesity.
Graphic abstract
中文翻译：
皂苷负责金合欢的抗肥胖活性
Acacia concinna (Willd.) DC。是一种主要来自东南亚的药用植物。Acacia concinna ( A. concinna )的豆荚是治疗或预防肥胖症的潜在候选者；然而，这些药用特性尚未得到详细研究。在这项研究中，A. concinna 中的抗肥胖化合物豆荚进行了调查。由胰脂肪酶抑制活性引导的豆荚提取物的色谱分离导致皂苷的分离。皂角苷的分解分析表明它们的化学成分为阿拉伯糖、单萜和五种糖类（葡萄糖、木糖、鼠李糖、奎诺糖、阿拉伯糖）。LC-MS 分析证实了分解分析中皂苷的预测结构，表明这些皂苷是单萜和糖单元的各种衍生物的混合物。这些皂苷以 IC 50强烈抑制胰脂肪酶活性7.9 μg/mL，并且在 6.3 μg/mL 时减少了 3T3-L1 脂肪细胞中的脂质积累。皂苷还通过介导蛋白激酶 A 和细胞外信号调节激酶途径的活性，以 3.1 或 6.3 μg/mL 增强 3T3-L1 脂肪细胞的脂解作用，这表明这种机制是观察到的脂肪细胞脂质含量减少的部分原因。结果强调，A. concinna是未来治疗和预防肥胖的抗致肥胖候选物的潜在来源。
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