DNA barcode datasets are a useful tool for conservation and aid in taxonomic identification, particularly in megadiverse tropical countries seeking to document and describe its biota, which is dropping at an alarming rate during recent decades. Here we report the barcodes for several low elevation bird species from northern Colombia with the goal to provide tools for species identification in this region of South America. We blood-sampled birds in a lowland tropical forest with various degrees of intervention using standard 3 × 12 m mist-nets. We extracted DNA and sequenced the COI barcode gene using standard primers and laboratory methods. We obtained 26 COI sequences from 18 species, 10 families and three orders and found that barcodes largely matched (but not always) phenotypic identification (> 90%) and they also facilitated the identification of several challenging passerine species. Despite our reduced sampling, our study represents the first attempt to document COI barcodes for birds (from blood samples) in this part of Colombia, which fills a considerable gap of sampling in this part of South America.

中文翻译：

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哥伦比亚北部鸟类的DNA条码

DNA条码数据集是保护和帮助分类识别的有用工具，特别是在试图记录和描述其生物群的巨型热带国家，这种生物群在最近几十年中以惊人的速度下降。在这里，我们报告了来自哥伦比亚北部的几种低海拔鸟类的条形码，目的是为南美地区的物种识别提供工具。我们使用标准的3×12 m薄雾网对低地热带森林中的鸟类进行了不同程度的采血。我们提取了DNA，并使用标准引物和实验室方法对COI条码基因进行了测序。我们从18个物种，10个科和三个顺序中获得了26个COI序列，发现条形码在很大程度上（但并非总是）与表型识别相匹配（> 90％），它们还有助于鉴定几种具有挑战性的雀形目物种。尽管我们减少了采样，但我们的研究还是首次尝试记录哥伦比亚该地区鸟类（来自血液样本）的COI条码，这填补了南美这一地区相当大的采样空白。