ABSTRACT

Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) causes destructive wilt disease to banana cultivation. However, knowledge of mechanisms in virulence regulation of Foc TR4 is still inadequate. In our previous work, two hypothetical FocCP1 proteins that belong to the cerato-platanin (CP) family were identified in the secretome of Foc TR4. In the present study, knock-out mutants of two FocCP1 were constructed and functions of two proteins were investigated. The results showed that the two FocCP1 play important roles in regulation of full virulence but are not required for vegetative growth or conidiation of the pathogen; besides, there is a functional redundancy between the two FocCP1. Moreover, we found that FocCP1 proteins interact with MaPR1 proteins through yeast two-hybrid and BiFC test. Our results suggest that FocCP1 proteins are important for the virulence of Foc TR4 and might inhibit the antifungal activity of MaPR1 by directly interacting with them.

摘要

尖孢镰刀菌f。sp。立方体热带种族4（Foc TR4）对香蕉栽培造成破坏性的枯萎病。但是，对Foc TR4的毒力调节机制的认识仍然不足。在我们以前的工作中，在Foc TR4的分泌组中鉴定了两个属于cerato-platanin（CP）家族的假想FocCP1蛋白。在本研究中，构建了两个FocCP1的敲除突变体，并研究了两种蛋白质的功能。结果表明，两种FocCP1在完全毒力的调节中起着重要作用，但对于病原体的营养生长或分生却不是必需的。此外，两个FocCP1之间存在功能冗余。此外，我们发现FocCP1蛋白通过酵母双杂交和BiFC测试与MaPR1蛋白相互作用。