Abstract

The aim of this study was to evaluate cytogenotoxicity in mammalian cells induced by ingestion of superficial water from SESS. For this purpose, surface water was collected from two points of SESS: São Vicente Channel (SVC) and Piaçaguera Channel (PIC). Four groups (n = 5) of adult male Wistar (8 weeks old) received for 5 days: (a) filtered tap water (water control), (b) tap water with 2.4% of NaCl (saline control), (c) estuarine water from PIC and (d) estuarine water from SVC. Results demonstrated that Ki67 immunoexpression was higher in hepatocytes exposed to both sampling site, while caspase-3 demonstrated downregulation in rat liver exposed to estuarine water. There was also significant increase in micronuclei frequency in bone marrow cells and hepatocytes, and DNA damage in blood and liver of rats exposed to estuarine water from SVC and PIC. In summary, studies with complex mixtures, such as contaminated estuarine water are important since this work confirmed by experiments using in vivo mammalian cells of rats that SESS water are genotoxic, mutagenic and cytotoxic, denoting concern for environmental health.

摘要

这项研究的目的是评估由SESS摄入表层水诱导的哺乳动物细胞的细胞遗传毒性。为此，从SESS的两个点收集了地表水：圣维森特河（SVC）和皮亚古拉河（PIC）。四组（n = 5）成年雄性Wistar（8周龄）接受了5天：（a）过滤自来水（水控制），（b）含2.4％NaCl的自来水（盐水控制），（c） PIC的河口水和（d）SVC的河口水。结果表明，暴露于两个采样位点的肝细胞中Ki67免疫表达较高，而caspase-3在暴露于河口水的大鼠肝脏中显示出下调。暴露于SVC和PIC的河口水的大鼠的骨髓细胞和肝细胞中的微核频率也显着增加，血液和肝脏中的DNA损伤也明显增加。总而言之，对复杂混合物（例如受污染的河口水）的研究非常重要，因为这项工作已通过使用大鼠体内哺乳动物细胞的实验证实，SESS水具有遗传毒性，诱变性和细胞毒性，这表明了对环境健康的关注。