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Use of Liquid Scintillation Counting to Study the Fate of C14 Labeled Glyphosate in Entisol Soil System
Communications in Soil Science and Plant Analysis ( IF 1.3 ) Pub Date : 2021-05-21 , DOI: 10.1080/00103624.2021.1925690
Aman D. Sharma 1 , Balwant Singh 2 , Rai Kookana 3
Affiliation  

ABSTRACT

Due to reduced tillage practices, glyphosate is widely used as a herbicide in grain farming systems of Australia. Residual glyphosate when mixed with water bodies can be a health concern. Updated information concerning the fate of glyphosate in soil systems with biochar amendment is lacking. For this experiment, different biochars were weighed and premixed in 2 ml Eppendorf tubes. A solution (Mixture of mercuric chloride and radiolabeled C14 glyphosate) having different concentrations provided by the commonwealth scientific and industrial research organization was used. Tubes having solutions were shaken on end over end shaker for 24 h. Tubes were removed from the shaker and centrifuged for 5 min. at 13000 revolutions per minute. Digested, labeled samples after counting disintegration per minute on Tricarb 4810 were used as sorption data. Results showed significant adsorption of glyphosate in the Entisol soil systems in the presence of char. The results have been published, however, the author wants to communicate in detail the role of liquid scintillation counting as a future research method for radiolabeled herbicides to understand the adsorption behavior of herbicides like glyphosate in different soil systems.



中文翻译:

使用液体闪烁计数研究 C14 标记草甘膦在 Entisol 土壤系统中的命运

摘要

由于减少耕作方式,草甘膦在澳大利亚的谷物种植系统中被广泛用作除草剂。与水体混合时残留的草甘膦可能会引起健康问题。缺乏关于草甘膦在使用生物炭改良剂的土壤系统中的归宿的最新信息。对于该实验,称重不同的生物炭并在 2 ml Eppendorf 管中预混合。溶液(氯化汞和放射性标记的 C 14 的混合物)草甘膦)使用了由英联邦科学和工业研究组织提供的不同浓度。将装有溶液的试管在端对端振荡器上振荡 24 小时。从振荡器中取出试管并离心 5 分钟。每分钟 13000 转。在 Tricarb 4810 上计算每分钟崩解后的消化标记样品用作吸附数据。结果表明,在炭存在的情况下,Entisol 土壤系统中草甘膦有显着吸附。结果已经发表,然而,作者想详细交流液体闪烁计数作为放射性标记除草剂的未来研究方法的作用,以了解草甘膦等除草剂在不同土壤系统中的吸附行为。

更新日期:2021-05-21
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