An analytical miniaturized methodology based on solid-phase-microextraction (mini_SPME) followed by gas chromatography coupled to mass spectrometry (GC-MS) has been developed for the identification of volatile and semivolatile compounds in honey samples. The main influential experimental parameters, such as the type of fibre coating, extraction temperature, solvent addition, extraction mode, ionic strength, and sample dilution were optimized. A design of experiments (DOE) was conducted including twenty-four target compounds. The final extraction conditions comprised the use of 200 mg of honey mixed with 200 μL of water (100%, w/v), employing a DVB/CAR/PDMS fibre in the headspace mode at 100 °C for 30 min. The mini_SPME-GC-MS method was successfully validated in terms of linearity, repeatability, reproducibility and accuracy. Finally, it was applied to a broad range of varieties of real honey samples from Galicia (NW Spain), as well as some foreign honeys, demonstrating suitability.

已经开发了一种基于固相微萃取（mini_SPME）的气相色谱-质谱联用（GC-MS）的分析小型化方法，用于鉴定蜂蜜样品中的挥发性和半挥发性化合物。优化了主要的影响实验参数，如纤维涂层的类型，提取温度，溶剂添加，提取方式，离子强度和样品稀释度。进行了包括二十四种目标化合物的实验设计（DOE）。最终提取条件包括使用200 mg蜂蜜与200μL水（100％，w / v）混合，在100°C的顶空模式下使用DVB / CAR / PDMS纤维30分钟。mini_SPME-GC-MS方法已成功验证了线性，可重复性，可重复性和准确性。最后，