For a biofertilizer to promote plant growth after application, cell survival during storage must be ensured, while the carrier formulation and packaging are important factors during storage. This study aimed to optimize the ratio of rubber wood ash (RWA), decanter cake (DCC), rice husk ash (RHA), and spent coffee grounds (SCG) as a mixed carrier of biofertilizer of purple non-sulfur bacteria (PNSB), Rhodopseudomonas palustris and Rubrivivax gelatinosus. Different packaging materials, and different sealing methods were investigated. The phytotoxicity of PNSB biofertilizers in a solid form, and their effectiveness to enhance rice (Oryza sativa L.) seedling growth in paddy soil were also investigated in comparison with a liquid form. A mixed carrier of RWA, DCC, RHA and SCG at a ratio of 3:4:2:1 most effectively maintained viability. After 6-month storage at 25 ± 3 °C, PNSB population of a mixed carrier containing R. palustris at roughly 10 log CFU/g in nylon-linear low density polyethylene (nylon-LLDPE) bags that were vacuum sealed at 500 W decreased by only 3 log cycles compared with 7 log cycles in the control. The PNSB biofertilizers, each genus and a mixed culture at 1: 1, either in solid or liquid form at their optimal dilutions showed no phytotoxicity. However, the mixed carrier itself as a control was slightly toxic. At these optimal dilutions, all PNSB biofertilizers in both liquid and solid formulations significantly increased rice growth in paddy soil compared with controls. A suitable mix of agro-industrial waste and suitable packing prolonged the shelf-life and potency of PNSB biofertilizers.

为了使生物肥料在施用后能促进植物生长，必须确保储存过程中的细胞存活，而载体的配方和包装是储存过程中的重要因素。这项研究旨在优化橡胶木灰（RWA），de析器蛋糕（DCC），稻壳灰（RHA）和废咖啡渣（SCG）作为紫色非硫细菌（PNSB）生物肥料混合载体的比例，紫假单胞菌和Rubrivivax gelatinosus。研究了不同的包装材料和不同的密封方法。固态PNSB生物肥料的植物毒性及其对水稻的增效作用（Oryza sativa与液体形式相比，还研究了稻田中L.）幼苗的生长。RWA，DCC，RHA和SCG比例为3：4：2：1的混合载体最有效地保持了生存能力。在25±3°C下存放6个月后，PNSB种群中含有青枯菌（R. palustris）的混合载体在以500 W真空密封的尼龙线性低密度聚乙烯（nylon-LLDPE）袋中，当浓度约为10 log CFU / g时，与对照中的7 log周期相比，仅减少了3 log周期。PNSB生物肥料，每个属，以其最佳稀释度以固体或液体形式以1：1混合培养，没有任何植物毒性。但是，作为对照的混合载体本身是有毒的。在这些最佳稀释度下，与对照组相比，液体和固体配方中的所有PNSB生物肥料均显着提高了水稻在水稻土中的生长。适当混合农业工业废料和适当包装可以延长PNSB生物肥料的保质期和效力。