ABSTRACT

The objective of this work was to evaluate the effectiveness of ozone in the control of fungi and the degradation of fumonisins in maize and possible effects on the chemical composition of the product. Two cultivars of hybrid maize (30F53H and AS1581 PRO) were used. Initially, the saturation process of the porous medium with ozone was studied. The incidence of Fusarium verticillioides, Penicillium spp. and Aspergillus flavus; the suitability of ozone to detoxify grains contaminated by fumonisins and possible qualitative changes in the product were evaluated. Ozone was applied at an inlet ozone concentration of 13.5 mg L⁻¹ and flow rate of 1.0 L min⁻¹, in five exposure periods (0, 12, 24, 36, 48 and 60 h). The estimated daily intake of FB by horses from grains subjected to ozonation was obtained. For the hybrid AS1581PRO, the estimated saturation time and concentration were equivalent to 67.34 min and 5.91 mg L⁻¹, respectively. With regard to the hybrid 30F53H, the saturation time and concentration were 41.74 min and 8.72 mg L⁻¹, respectively. Ozone was an effective fungicidal agent. The use of a concentration of 13.5 mg L⁻¹ over a 24 h-treatment led to a reduction of 81.2 and 86.2% in the concentrations of fumonisin B1 and fumonisin B2, respectively. The estimated daily intake of fumonisins was reduced to 17.21 µg kg⁻¹_BW per day, corresponding to half of the acceptable daily intake for equines. Ozone did not affect the chemical composition of the grain. Ozone is effective in controlling fungi and as a detoxifying agent in maize contaminated by fumonisins.

摘要

这项工作的目的是评估臭氧在控制真菌和降解玉米中伏马菌素方面的有效性以及对产品化学成分的可能影响。使用了两个杂交玉米品种（30F53H 和 AS1581 PRO）。初步研究了臭氧对多孔介质的饱和过程。Fusarium v​​erticillioides，Penicillium spp的发病率。和黄曲霉；对臭氧对被伏马菌素污染的谷物进行解毒的适用性以及产品可能发生的质量变化进行了评估。臭氧以 13.5 mg L ^-1的入口臭氧浓度和 1.0 L min ^{-1 的}流速施加，在五个暴露期（0、12、24、36、48 和 60 小时）。获得了马从经过臭氧处理的谷物中估计的每日 FB 摄入量。对于混合型 AS1581PRO，​​估计的饱和时间和浓度分别相当于 67.34 分钟和 5.91 mg L ^-1。对于杂化30F53H，饱和时间和浓度分别为41.74 min和8.72 mg L ^-1。臭氧是一种有效的杀菌剂。在 24 小时处理中使用 13.5 mg L ^-1的浓度分别导致伏马菌素 B1 和伏马菌素 B2 的浓度降低了 81.2% 和 86.2%。伏马菌素的估计每日摄入量降至 17.21 µg kg ^-1 _BW每天，相当于马可接受的每日摄入量的一半。臭氧不影响谷物的化学成分。臭氧可有效控制真菌，并可作为被伏马菌素污染的玉米的解毒剂。