Bisphenol A (BPA) is used to produce plastic and plastic derived products in multitude of daily utensils, being one of the industrial compounds most widely used. This endocrine disrupting chemical (EDCs) is a well-known environmental pollutant released into the aquatic environment from industrial wastewater, sewage sludge or landfill leachate. Aromatases are considered potential targets of EDCs with characteristics that make them suitable biomarkers of exposure to their effects. The main objective of our study was to evaluate the expression of cyp19a aromatase as a toxicological endpoint after BPA exposure through the identification and assessment of alterations of the main cells responsible for cyp19a1a and cyp19a1b expression in the zebrafish ovary and brain using different concentrations of BPA in water. Immunohistochemistry was used to analyze the expression of these enzymes in female zebrafish exposed and not exposed to different concentrations of BPA (1, 10, 100 and 1000 μg / L) in water (n = 6/group) for 14 days. The results obtained in this study showed that the cyp19a aromatase system, involved in the synthesis of steroid compounds, is specially located in distinct oocyte stages in the ovary (cyp19a1a) and in radial glial cells of the brain (cyp19a1b). An overexpression of these aromatases was observed after BPA exposure in zebrafish, peaking from a concentration of 10 µg/L and showing to be good biomarkers of exposure to identify the early effects of low BPA concentrations. To our knowledge, this study is the first to localize and quantify the expression of cyp19a1a and cyp19a1b in the cells of brain and ovary after fish exposure to different BPA concentrations in water.

双酚 A (BPA) 用于生产多种日用器具中的塑料和塑料衍生产品，是应用最广泛的工业化合物之一。这种内分泌干扰化学品 (EDC) 是一种众所周知的环境污染物，从工业废水、污水污泥或垃圾渗滤液中释放到水生环境中。芳香酶被认为是 EDC 的潜在靶标，其特征使其成为暴露于其影响的合适生物标志物。我们研究的主要目的是通过鉴定和评估负责斑马鱼卵巢和大脑中 cyp19a1a 和 cyp19a1b 表达的主要细胞的改变，评估 cyp19a 芳香酶的表达作为 BPA 暴露后的毒理学终点，使用不同浓度的 BPA水。n  = 6/组）14 天。本研究中获得的结果表明，参与类固醇化合物合成的 cyp19a 芳香酶系统特别位于卵巢 (cyp19a1a) 和大脑的径向胶质细胞 (cyp19a1b) 中不同的卵母细胞阶段。在斑马鱼中观察到 BPA 暴露后这些芳香酶的过度表达，从 10 µg/L 的浓度开始达到峰值，并显示出暴露的良好生物标志物，以识别低 BPA 浓度的早期影响。据我们所知，这项研究首次对鱼类暴露于水中不同浓度的 BPA 后大脑和卵巢细胞中 cyp19a1a 和 cyp19a1b 的表达进行了定位和量化。