This study evaluated the influence of different quantities of artificial substrate on water quality and the performance of Litopenaeus vannamei in an integrated biofilm-biofloc culture system. Thus, three treatments were performed: the control, the treatment without the addition of artificial substrate; T200, the treatment with a 200 % increase in the lateral area of the tanks using artificial substrates; and T400, the treatment with a 400 % increase in the lateral area of the tanks using artificial substrates. The study was conducted in nine 800 L tanks over 60 days. The animals were stocked at an initial density of 300 shrimp.m⁻² (equivalent to 500 shrimp m^-3), with an initial weight of 1.27 g (± 0.48). Ammonia concentrations did not differ significantly between treatments (p > 0.05). Increasing the amount of substrate from 200 % to 400 % did not cause significant differences in the nitrite concentrations between these treatments. However, in the control treatment, nitrite remained high (above 20 mg.L^-1) for a long period, negatively affecting shrimp performance. Nitrate was lower in T400, indicating a more dynamic process in the nitrogen cycle when the quantity of artificial substrate increased. Weekly growth rates, final weight, survival, and productivity were higher in the treatments integrating biofilm and biofloc substrates and did not show significant differences between T200 and T400. The results demonstrate the importance of artificial substrates in enhancing the water quality in biofloc culture systems over the long term, mostly in terms of maintaining nitrite concentrations below levels toxic to L. vannamei. The performance of the shrimp and the improved water quality at the end of the study reflected the advantages provided by incorporating artificial substrates in shrimp biofloc culture.

这项研究评估了在生物膜-生物絮凝剂集成培养系统中，不同数量的人工底物对水质的影响以及凡纳滨对虾的性能。因此，进行了三种处理：对照，不添加人工底物的处理；和。T200，使用人造基材将水箱的侧面面积增加200％的处理；使用T400和T400，则使用人造基材的水箱侧面面积增加了400％的处理量。该研究在60天内在9个800 L的水箱中进行。放养动物时初始密度为300虾米^-2（相当于500虾米^-3），初始重量为1.27克（±0.48）。两种处理之间的氨浓度没有显着差异（p> 0.05）。将底物的量从200％增加到400％不会在这些处理之间引起亚硝酸盐浓度的显着差异。但是，在对照治疗中，亚硝酸盐仍然很高（高于20 mg.L ^-1）很长一段时间，对虾的性能造成负面影响。T400中的硝酸盐含量较低，这表明当人造底物的数量增加时，氮循环中的过程更加动态。在整合生物膜和生物絮凝物基质的处理中，每周生长率，最终重量，存活率和生产率更高，并且在T200和T400之间没有显示出显着差异。结果表明，从长远来看，人造底物对于提高生物絮凝培养系统中水质的重要性，主要体现在将亚硝酸盐浓度保持在对南美白对虾有毒的水平以下。研究结束时虾的性能和水质的改善反映了将人工底物掺入虾生物絮凝剂养殖中所提供的优势。