Ljungan virus (LV), a Parechovirus of the Picornavirus family, first isolated from a bank vole at the Ljungan river in Sweden, has been implicated in the risk for autoimmune type 1 diabetes. An assay for neutralizing Ljungan virus antibodies (NLVA) was developed using the original 87–012 LV isolate. The goal was to determine NLVA titres in incident 0–18 years old newly diagnosed type 1 diabetes patients (n=67) and school children controls (n=292) from Jämtland county in Sweden. NLVA were found in 41 of 67 (61 %) patients compared to 127 of 292 (44 %) controls (P=0.009). In the type 1 diabetes patients, NLVA titres were associated with autoantibodies to glutamic acid decarboxylase (GADA) (P=0.023), but not to autoantibodies against insulin (IAA) or islet antigen-2 (IA-2A). The NLVA assay should prove useful for further investigations to determine levels of LV antibodies in patients and future studies to determine a possible role of LV in autoimmune type 1 diabetes.

中文翻译：

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在新诊断的 1 型糖尿病儿童中中和 Ljungan 病毒抗体

Ljungan 病毒(LV) 是一种小核糖核酸病毒家族的副肠病毒，首次从瑞典 Ljungan 河的河岸田鼠中分离出来，与自身免疫性 1 型糖尿病的风险有关。使用最初的 87-012 LV 分离株开发了一种中和 Ljungan 病毒抗体 (NLVA) 的检测方法。目标是确定瑞典耶姆特兰县 0-18 岁新诊断 1 型糖尿病患者 ( n =67) 和学童对照 ( n =292) 的NLVA 滴度。与 292 名对照组中的 127 名 (44%) 相比，67 名 (61%) 患者中有 41 名 ( P = 0.009) 发现了 NLVA。在 1 型糖尿病患者中，NLVA 滴度与谷氨酸脱羧酶 (GADA) 自身抗体相关。P = 0.023），但不是针对胰岛素（IAA）或胰岛抗原2（IA-2A）的自身抗体。NLVA 测定应证明对确定患者 LV 抗体水平的进一步调查和未来研究以确定 LV 在自身免疫性 1 型糖尿病中的可能作用有用。