Dendritic cells (DCs) play an essential role in the initiation of adaptive immune responses, but they are rare in all organs. The traditional methods used to increase the yield and purity of DCs are the early removal of granulocyte culture medium and the isolation of high-purity DCs by magnetic-activated cell sorting (MACS). This study provides a more rapid and economical optimization method to obtain more high-purity DCs. (i) We harvested 18% more bone marrow (BM) cells by using forceps to crack the epiphysis instead of cutting it with scissors during BM cell extraction. (ii) When the cells in the culture medium that is discarded on day 3 in the traditional method were centrifuged and then added back to the petri dish, the DC yield on day 5 increased by 61%. (iii) On the third day, the addition of fresh medium and the retention of the original medium rather than discarding it increased the number of DCs harvested on the fifth day by 137%. (i-iii) The improved method cost an average of 74% less than the conventional method and yielded the same number and function of cells. (iv) The initial number of BM cells was increased by 15% in 4-week-old mice compared with 8-week-old mice. (v) The Percoll density centrifugation (PDS) method was used to purify DCs on day 6 after induction, and the purity of the DCs was greater than 90%, which showed no significant difference from the MACS method. However, the yield of the PDS method increased by 21%. In addition, the PDS method has a lower cost, with an average purification cost of 4 CNY ($0.58) compared with 648 CNY ($93.25) for MACS, reducing the cost by 99%. Therefore, high-purity and high-yield DCs can be rapidly obtained through a five-step improvement in the process of BM cell extraction, induction and purification.

树突状细胞 (DC) 在适应性免疫反应的启动中起重要作用，但它们在所有器官中都很罕见。用于提高 DCs 产量和纯度的传统方法是早期去除粒细胞培养基和通过磁激活细胞分选 (MACS) 分离高纯度 DCs。本研究为获得更多高纯度DC提供了一种更快速、更经济的优化方法。(i) 在 BM 细胞提取过程中，我们通过使用镊子裂开骨骺而不是用剪刀切割骨骺，多收获了 18% 的骨髓 (BM) 细胞。(ii) 将传统方法第 3 天丢弃的培养基中的细胞离心后重新加入培养皿中，第 5 天的 DC 产量增加了 61%。(iii) 第三天，添加新鲜培养基并保留原始培养基而不是丢弃它使第五天收获的 DC 数量增加了 137%。(i-iii) 改进方法的成本平均比传统方法低 74%，并产生相同数量和功能的细胞。(iv) 与 8 周龄小鼠相比，4 周龄小鼠的 BM 细胞初始数量增加了 15%。(v) 诱导后第6天采用Percoll密度离心(PDS)法纯化DCs，DCs纯度大于90%，与MACS法无显着差异。然而，PDS 方法的产率增加了 21%。此外，PDS法成本更低，平均纯化成本为4元（0.58美元），而MACS为648元（93.25美元），成本降低99%。所以，