The present study investigated the effect of Trigonella foenum-graecum extract (TFGE) on GH3 and HEK293 pituitary adenoma cell growth in vitro in mice model in vivo and evaluated the underlying mechanism. TFGE exposure in dose-dependent manner suppressed the viability of GH3 and HP75 cells at 5–30 µM concentrations. Treatment of GH3 and HP75 cells with 30 µM TFGE inhibited colony formation significantly (P < .05) compared to the un-exposed cells. In GH3 and HP75 cells, TFGE exposure suppressed invasion potential significantly (P < .05) at 5 and 30 µM concentrations. TFGE treatment at 5 and 30 µM for 72 h significantly (P < .05) elevated induction of apoptosis. The H19 expression was significantly (P < .05) inhibited by TFGE-treatment in GH3 and HEK293 cells. TFGE treatment at 2.5 and 10 mg/kg doses led to a significantly (P < .05) suppression in the tumor growth in HP75 cell-implanted mice. TFGE treatment of GH3 and HP75 cells at 5 and 30 µM down-regulated 4E-BP1 activation. TFGE treatment inhibits GH3 and HEK293 cell viability and inhibits tumor development. It suppresses H19 expression and upregulates 4E-BP1 phosphorylation in GH3 and HEK293 cells. Therefore, TFGE may be used for the treatment of pituitary adenoma.

本研究在体内模型中研究了胡芦巴提取物（TFGE）对GH3和HEK293垂体腺瘤细胞体外生长的影响，并评估了其潜在机制。TFGE暴露以剂量依赖性方式抑制了5–30 µM浓度的GH3和HP75细胞的活力。 与未暴露的细胞相比，用30 µM TFGE处理GH3和HP75细胞可显着抑制集落形成（P <.05）。在GH3和HP75细胞中，TFGE暴露 在5和30 µM浓度下显着抑制了侵袭潜能（P <.05）。TFGE在5和30 µM下处理72 h显着（P <.05）诱导凋亡增加。 在GH3和HEK293细胞中，TFGE处理显着抑制了H19的表达（P <0.05）。2.5和10 mg / kg剂量的TFGE处理可显着（P  <.05）抑制HP75细胞植入小鼠的肿瘤生长。TFGE处理5和30 µM的GH3和HP75细胞下调了4E-BP1的活化。TFGE处理可抑制GH3和HEK293细胞的活力，并抑制肿瘤的发展。它抑制GH3和HEK293细胞中的H19表达并上调4E-BP1磷酸化。因此，TFGE可用于垂体腺瘤的治疗。