The salivary glands are closely associated with stress conditions and the neuroendocrine system. The concentrations of these salivary biomarkers fluctuate with a person’s disease status. Until now, salivary diagnostics have been promising; however, the analytical tools for detecting salivary biomarkers have remained burdensome due to the lack of user-friendly and cost-effective equipment. In this study, we used a microfluidic starch-coated paper-based chip to isolate ɑ-amylase from human saliva. The concentration of ɑ-amylase on the head and tail sections of the paper-based chip was compared in the presence or absence of starch. We found that ɑ-amylase was highly concentrated only in the head section of the starch-coated paper-based chip through Western blotting (WB) and enzyme-linked immunosorbent assay (ELISA). We expect our designed paper-based chip will aid in α-amylase detection technologies using saliva and small amounts of other biofluids including blood, tears, pancreatic juice, and urine.

唾液腺与压力状况和神经内分泌系统密切相关。这些唾液生物标志物的浓度随人的疾病状态而波动。到目前为止，唾液诊断一直很有希望。然而，由于缺乏用户友好且具有成本效益的设备，用于检测唾液生物标志物的分析工具仍然很繁重。在这项研究中，我们使用微流控淀粉涂层纸基芯片从人类唾液中分离 ɑ-淀粉酶。在存在或不存在淀粉的情况下，比较了纸基芯片的头部和尾部部分 ɑ-淀粉酶的浓度。我们通过蛋白质印迹 (WB) 和酶联免疫吸附试验 (ELISA) 发现，ɑ-淀粉酶仅在淀粉涂层纸基芯片的头部高度浓缩。