The contamination of food and drinking water by bacteria that cause food poisoning is a critical public health issue. In general, the procedures for testing the presence of pathogenic bacteria in public is not very effective, because it is time consuming and does not facilitate real-time monitoring in a field setting. Therefore, this study introduced a fully automated platform that would allow non-experts to easily examine the major food poisoning bacteria in a field setting, from pretreatment samples to final detection. Two enterobacteria species, of four different bacteria species (i.e., Escherichia coli and Salmonella typhimurium) were successfully immobilized on the surface of magnetic beads by exploiting the specific binding force with mannose. Subsequently, magneto-electrochemical impedance measurement technology allowed bacterial detection with a high sensitivity. E. coli, typical enterobacteria, was detected in 25 min with a detection limit of 100 CFU/mL. Moreover, we demonstrated that the automatic performance and the experimental consistency was greatly improved in comparison with those of manually conducted experiments.

导致食物中毒的细菌对食物和饮用水的污染是一个重要的公共卫生问题。一般而言，在公共场所检测病原菌的程序不是很有效，因为它耗时且不利于现场设置的实时监测。因此，本研究引入了一个全自动平台，允许非专家轻松检查现场环境中的主要食物中毒细菌，从预处理样本到最终检测。两种肠杆菌属，四种不同的细菌种类（即大肠杆菌和鼠伤寒沙门氏菌）) 通过利用与甘露糖的特异性结合力成功地固定在磁珠表面。随后，磁电化学阻抗测量技术允许以高灵敏度进行细菌检测。大肠杆菌是典型的肠杆菌，可在 25 分钟内检测到，检测限为 100 CFU/mL。此外，我们证明与手动进行的实验相比，自动性能和实验一致性得到了极大的提高。